Heading which way? Y-maze chemical assays: not all crustaceans are alike
Heading which way? Y-maze chemical assays: not all crustaceans are alike
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DOI：
10.1007/s10152-015-0435-6
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发表时间：
2015-09-01
影响因子：
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通讯作者：
Harzsch, Steffen
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文献类型：
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作者：
Kenning, Matthes;Lehmann, Philipp;Harzsch, Steffen
在一个充满化学物质的世界里，许多甲壳类动物依靠复杂的嗅觉系统来指导与寻找食物有关的行为，或者评估同种动物和捕食者的存在。我们分析了等足类Saduria entomon的一系列刺激的动物可能会遇到在其自然栖息地使用Y-迷宫生物测定的反应。为了证明实验设计的有效性，使用相同的生物测定来测试小龙虾Procambarus fallax的行为，其追踪气味的能力得到了很好的证明。小龙虾在Y型迷宫中表现良好，能够高度准确地定位与食物相关的气味的来源。等足目S.昆虫对大多数刺激反应冷淡或厌恶。在1800次试验中，15种不同的刺激物中只有4种产生了统计学上显著的结果，只有一种气味被发现具有显著的吸引力。研究结果提出了几个问题，无论是提出的刺激和/或使用的实验设置代表了生态相关的情况下，S。昆虫。在每一种情况下，我们的实验都表明，既定的方法不能轻易地从一个物种转移到另一个物种。
In a world full of chemicals, many crustaceans rely on elaborate olfactory systems to guide behaviors related to finding food or to assess the presence of conspecifics and predators. We analyzed the responses of the isopod Saduria entomon to a range of stimuli by which the animal is likely to encounter in its natural habitat using a Y-maze bioassay. In order to document the efficiency of the experimental design, the same bioassay was used to test the behavior of the crayfish Procambarus fallax whose ability to track odors is well documented. The crayfish performed well in the Y-maze and were able to locate the source of a food-related odor with high fidelity. The isopod S. entomon reacted indifferently or with aversion to most of the stimuli applied. In 1800 trials, only four out of 15 different stimuli yielded statistically significant results, and only one odorant was found to be significantly attractive. The findings raise several questions whether the stimuli presented and/or the experimental setup used represents an ecologically relevant situation for S. entomon. In each instance, our experiments illustrate that established methods cannot be readily transferred from one species to another.