Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants.
Multifactorial and Species-Specific Feedback Regulation of the RNA Surveillance Pathway Nonsense-Mediated Decay in Plants.
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DOI：
10.1093/pcp/pcz141
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发表时间：
2019-09
影响因子：
4.9
通讯作者：
Anil K. Kesarwani;Hsin-Chieh Lee;Patrizia G Ricca;G. Sullivan;Natalie Faiss;Gabriele Wagner;Anna Wunderling;Andreas Wachter
中科院分区：
文献类型：
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作者：
Anil K. Kesarwani;Hsin-Chieh Lee;Patrizia G Ricca;G. Sullivan;Natalie Faiss;Gabriele Wagner;Anna Wunderling;Andreas Wachter
无义介导的衰变（NMD）是一种RNA监视机制，其检测异常的转录物特征，并触发错误的以及生理性RNA的降解。NMD最初被认为是结构性的，现在被认为是对内在信号和各种压力的严格控制。为了更好地了解NMD调控及其功能意义，我们系统地研究了两个双子叶植物和一个单子叶植物的中央NMD组件的反馈控制。基于对转录特征、周转率和稳态水平的分析，UPF1、UPF3和SMG 7在两种双子叶植物中都处于反馈控制之下，而UPF2不在反馈控制之下。在本研究中研究的单子叶植物中，仅SMG7在NMD抑制后被轻微诱导。拟南芥内源NMD因子蛋白的检测证实了SMG7含量与NMD活性呈负相关。此外，有证据表明SMG7是UPF1去磷酸化所必需的。我们对植物NMD反馈控制的全面和比较研究揭示了这种RNA监视途径的复杂和物种特异性衰减，对NMD在生理和应激反应中的众多功能具有重要意义。
Nonsense-mediated decay (NMD) is an RNA surveillance mechanism that detects aberrant transcript features, and triggers degradation of erroneous as well as physiological RNAs. Originally considered to be constitutive, NMD is now recognized to be tightly controlled in response to inherent signals and diverse stresses. To gain a better understanding of NMD regulation and its functional implications, we systematically examined feedback control of the central NMD components in two dicot and one monocot species. Based on the analysis of transcript features, turnover rates, and steady state levels, UPF1, UPF3, and SMG7, but not UPF2 are under feedback control in both dicots. In the monocot investigated in this study, only SMG7 was slightly induced upon NMD inhibition. Detection of the endogenous NMD factor proteins in Arabidopsis thaliana substantiated a negative correlation between NMD activity and SMG7 amounts. Furthermore, evidence was provided that SMG7 is required for the dephosphorylation of UPF1. Our comprehensive and comparative study of NMD feedback control in plants reveals complex and species-specific attenuation of this RNA surveillance pathway, with critical implications for the numerous functions of NMD in physiology and stress responses.