An application of the alkaline extraction - glucoamylase hydrolysis method to analyze starch and sugar contents of bamboo
An application of the alkaline extraction - glucoamylase hydrolysis method to analyze starch and sugar contents of bamboo
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DOI：
10.1007/s10086-004-0674-7
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发表时间：
2005-01-01
影响因子：
2.9
通讯作者：
Imamura, Y
中科院分区：
文献类型：
--
作者：
Okahisa, Y;Yoshimura, T;Imamura, Y
采用碱提-糖化酶水解（AG）法（用氢氧化钠提取糖和淀粉，用糖化酶和α-淀粉酶水解淀粉）和乙醇提取-高氯酸水解（EPA）法对毛竹（Phyllostachys pubescens Mazel）的糖和淀粉含量进行了分析比较。结果表明，两种方法提取毛竹中游离糖的能力相当。但在适宜条件下，AG法比EPA法更能准确地分析毛竹中的淀粉。当我们考虑到AG法比EPA法更好的时间性能时，我们可以得出结论，AG法在分析毛竹糖和淀粉含量方面优于EPA法上级。
The alkaline extraction - glucoamylase hydrolysis (AG) method, which extracts sugars and starch by using sodium hydroxide and hydrolyzes starch by using glucoamylase and a-amylase, was compared with the established ethanol extraction - perchloric acid hydrolysis (EPA) method to analyze the sugar and starch contents of moso bamboo (Phyllostachys pubescens Mazel). The results suggested that the two methods had comparable abilities to extract free sugars from moso bamboo. However, the AG method analyzed starch in moso bamboo more accurately than the EPA method under the proper conditions. When we take into account the better time performance of the AG method versus the EPA method, we can conclude that the AG method is superior to EPA for analyzing the sugar and starch contents of moso bamboo.