Determination of the distribution of light, optical properties, drug concentration, and tissue oxygenation in-vivo in human prostate during motexafin lutetium-mediated photodynamic therapy
Determination of the distribution of light, optical properties, drug concentration, and tissue oxygenation in-vivo in human prostate during motexafin lutetium-mediated photodynamic therapy
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DOI：
10.1016/j.jphotobiol.2004.09.013
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发表时间：
2005-06-01
影响因子：
5.4
通讯作者：
Hahn, SM
中科院分区：
文献类型：
--
作者：
Zhu, TC;Finlay, JC;Hahn, SM
在光动力疗法治疗前列腺癌中，需要对光通量率的分布、光学特性、药物浓度和组织氧合进行定量分析。我们已经开发了一个集成的系统，以确定这些数量之前和之后PDT治疗使用机动探头。利用组织间质各向同性探测器测量了人前列腺癌组织的光学特性（吸收系数、传输系数、散射系数和有效衰减系数）。在沿着沿着每个导管的不同位置处，在莫特沙芬镥（MLu）介导的PDT之前和之后，在732 nm处进行测量。光动力治疗期间，还测量了沿着导管的光通量率分布。使用白色光源的漫吸收光谱测量允许外推氧饱和度NOD、总血容量（[Hb]（t））和MLu浓度的分布。药物浓度的分布也进行了研究，使用荧光从一个单一的光纤，并被发现是在良好的协议与吸收光谱法确定的值。这项研究表明，显着的组织光学特性和MLu药物分布的前列腺间和前列腺内的变化，这表明，在治疗过程中监测这些值的实时剂量测量和反馈系统，应考虑在未来的PDT研究。(c)2004 Elsevier B.V.保留所有权利。
It is desirable to quantify the distribution of the light fluence rate, the optical properties, the drug concentration, and the tissue oxygenation for photodynamic therapy (PDT) of prostate cancer. We have developed an integrated system to determine these quantities before and after PDT treatment using motorized probes. The optical properties (absorption (mu(a) transport scattering and effective attenuation (mu(eff)) coefficients) of cancerous human prostate were measured in-vivo using interstitial isotropic detectors. Measurements were made at 732 nm before and after motexafin lutetium (MLu) mediated PDT at different locations along each catheter. The light fluence rate distribution was also measured along the catheters during PDT. Diffuse absorption spectroscopy measurement using a white light source allows extrapolation of the distribution of oxygen saturation NOD, total blood volume ([Hb](t)), and MLu concentration. The distribution of drug concentration was also studied using fluorescence from a single optical fiber, and was found to be in good agreement with the values determined by absorption spectroscopy. This study shows significant inter- and intra-prostatic variations in the tissue optical properties and MLu drug distribution, suggesting that a real-time dosimetry measurement and feedback system for monitoring these values during treatment should be considered in future PDT studies. (c) 2004 Elsevier B.V. All rights reserved.