Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice.
Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice.
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水稻抗病增强病斑模拟突变体斑点叶 5 的蛋白质组学分析
DOI：
10.1186/1939-8433-6-1
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发表时间：
2013-01-07
期刊：
影响因子：
--
通讯作者：
Ma B
中科院分区：
文献类型：
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作者：
Chen X;Fu S;Zhang P;Gu Z;Liu J;Qian Q;Ma B
背景水稻（Oryza sativa L.）斑点叶5（spl 5）显示抗病性增强的表型，表明SPL 5负调节细胞死亡和抗性应答。为了了解SPL 5突变诱导细胞死亡和抗性反应的分子机制，采用蛋白质组学方法鉴定了突变体和野生型之间的差异蛋白质。结果双向凝胶电泳的蛋白质组学数据显示，14个候选蛋白在突变体中表达显著上调或下调。这些蛋白质参与多种生物过程，包括前体mRNA剪接、氨基酸代谢、光合作用、糖酵解、活性氧（ROS）代谢和防御反应。通过qPCR和酶活性测定进一步分析了具有显著上调inspl 5的两种候选蛋白-APX 7（一种关键的ROS代谢酶）和Chia 2a（一种发病相关蛋白）。结论在spl 5突变体的损伤形成过程中，APX 7和Chia 2a的转录水平和酶活性均受到明显的诱导。通常情况下，insl 5的上调蛋白参与防御反应或PCD，而下调蛋白则参与氨基酸代谢和光合作用。这些结果可能有助于对spl 5诱导植物细胞死亡和抗病性的分子机制有新的认识。
BackgroundA lesion-mimic mutant in rice (Oryza sativaL.),spotted leaf 5(spl5), displays a disease-resistance-enhanced phenotype, indicating that SPL5 negatively regulates cell death and resistance responses. To understand the molecular mechanisms ofSPL5mutation-induced cell death and resistance responses, a proteomics-based approach was used to identify differentially accumulated proteins between thespl5mutant and wild type (WT).ResultsProteomic data from two-dimensional gel electrophoresis showed that 14 candidate proteins were significantly up- or down-regulated in thespl5mutant compared with WT. These proteins are involved in diverse biological processes including pre-mRNA splicing, amino acid metabolism, photosynthesis, glycolysis, reactive oxygen species (ROS) metabolism, and defense responses. Two candidate proteins with a significant up-regulation inspl5– APX7, a key ROS metabolism enzyme and Chia2a, a pathogenesis-related protein – were further analyzed by qPCR and enzyme activity assays. Consistent with the proteomic results, both transcript levels and enzyme activities of APX7 and Chia2a were significantly induced during the course of lesion formation inspl5leaves.ConclusionsMany functional proteins involving various metabolisms were likely to be responsible for the lesion formation ofspl5mutant. Generally, inspl5, the up-regulated proteins involve in defense response or PCD, and the down-regulated ones involve in amino acid metabolism and photosynthesis. These results may help to gain new insight into the molecular mechanism underlyingspl5-induced cell death and disease resistance in plants.