Interaction Between Conjunctival Epithelial Cells and Mast Cells Induces CCL2 Expression and Piecemeal Degranulation in Mast Cells
Interaction Between Conjunctival Epithelial Cells and Mast Cells Induces CCL2 Expression and Piecemeal Degranulation in Mast Cells
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DOI：
10.1167/iovs.12-10664
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发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Matsuda, Akira
中科院分区：
文献类型：
--
作者：
Iwamoto, Satoshi;Asada, Yosuke;Matsuda, Akira
目的.在从患有特应性角膜结膜炎（AKC）/春季角膜结膜炎（VKC）的患者获得的巨大乳头组织样品中观察到上皮内肥大细胞。我们研究了结膜上皮细胞和肥大细胞之间的相互作用。利用共培养模型研究了人肥大细胞和结膜上皮细胞（HCjE）之间的相互作用。蛋白质阵列分析，ELISA和实时PCR进行测试的相互作用。切除巨乳头组织样本（n = 6）用于治疗目的，并进行免疫组织化学分析CCL 2的表达。重组CCL 2（10 ng/mL）与培养的人肥大细胞反应，并进行超微结构分析。用豚草（RW）诱导的小鼠实验性变应性结膜炎模型来检测ccl 2 mRNA表达和肥大细胞形态。蛋白质芯片和实时荧光定量PCR分析表明，肥大细胞-HCjE共培养诱导了CCL 2蛋白/mRNA的表达。肥大细胞中观察到CCL 2 mRNA上调，而免疫组织化学法观察到巨乳头结膜上皮中原位CCL 2表达。超微结构分析表明，重组CCL 2治疗诱导的零碎脱粒（PMD）的肥大细胞。巨乳头组织的超微结构分析显示结膜上皮细胞内的肥大细胞PMD。结论：RW诱导的实验性变态反应性结膜炎模型中，结膜组织中ccl 2 mRNA表达增加，PMD形态学改变。肥大细胞-结膜上皮细胞相互作用诱导CCL 2表达和随后的PMD。
PURPOSE. Intraepithelial mast cells are observed in giant papillae tissue samples obtained from patients with atopic keratoconjunctivitis (AKC)/vernal keratoconjunctivitis (VKC). We examined the roles of interaction between the conjunctival epithelial cells and mast cells.METHODS. The interaction between human mast cells and conjunctival epithelial cells (HCjE) was investigated using a coculture model. Protein array analysis, ELISA, and real-time PCR were performed to test the interaction. Tissue samples (n = 6) from giant papillae were resected for therapeutic purposes, and subjected to immunohistological analysis of CCL2 expression. Recombinant CCL2 (10 ng/mL) was reacted with the cultured human mast cells and ultrastructural analysis was performed. A ragweed (RW)-induced mouse experimental allergic conjunctivitis model was used to examine ccl2 mRNA expression and mast cell morphology.RESULTS. Protein array and real-time PCR analyses showed that CCL2 protein/mRNA expression was induced by mast cell-HCjE coculture. Upregulation of CCL2 mRNA was observed in mast cells, whereas in situ CCL2 expression was observed at the conjunctival epithelium of the giant papillae by immunohistochemistry. Ultrastructural analysis showed that recombinant CCL2 treatment induced piecemeal degranulation (PMD) in the mast cells. Ultrastructural analysis of tissues from the giant papillae showed PMD of mast cells within the conjunctival epithelial cells. The RW-induced experimental allergic conjunctivitis model showed increased ccl2 mRNA expression and PMD morphology in the conjunctivae.CONCLUSIONS. Mast cell-conjunctival epithelial cell interaction induces CCL2 expression and subsequent PMD.