Graphene Bioelectronic Nose for the Detection of Odorants with Human Olfactory Receptor 2AG1
Graphene Bioelectronic Nose for the Detection of Odorants with Human Olfactory Receptor 2AG1
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DOI：
10.3390/chemosensors9070174
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发表时间：
2021-07-01
期刊：
影响因子：
4.2
通讯作者：
Guy, Owen J.
中科院分区：
文献类型：
--
作者：
Goodwin, Danielle M.;Walters, Ffion;Guy, Owen J.
利用人嗅觉受体2AG 1（OR 2AG 1），一种由七个跨膜结构域组成的G蛋白偶联受体（GPCR），固定在石墨烯电阻器上，用于检测丁酸戊酯（AB）的实时传感器被证明。使用CVD石墨烯作为传感器平台，允许比基于碳纳米管、金或氧化石墨烯平台的类似传感器更灵敏的检测的更大潜力。通过将1，5二氨基萘（DAN）非共价π-π堆叠到石墨烯通道上，并随后使用戊二醛偶联将OR 2AG 1受体锚定到DAN分子上，制造和修饰特定的石墨烯电阻器传感器。目标气味剂丁酸戊酯和OR 2AG 1受体蛋白之间的结合产生石墨烯电阻器传感器的电阻变化。官能化石墨烯电阻器传感器表现出在0.1-500 pM之间的线性传感器响应，并且对丁酸戊酯具有高选择性，灵敏度低至500 fM，而使用非特异性酯的对照测量产生可忽略的传感器响应。这里描述的方法提供了一种可用于生物电子鼻应用的替代感测平台。
A real-time sensor for the detection of amyl butyrate (AB) utilising human olfactory receptor 2AG1 (OR2AG1), a G-protein coupled receptor (GPCR) consisting of seven transmembrane domains, immobilized onto a graphene resistor is demonstrated. Using CVD graphene as the sensor platform, allows greater potential for more sensitive detection than similar sensors based on carbon nanotubes, gold or graphene oxide platforms. A specific graphene resistor sensor was fabricated and modified via non-covalent pi-pi stacking of 1,5 diaminonaphthalene (DAN) onto the graphene channel, and subsequent anchoring of the OR2AG1 receptor to the DAN molecule using glutaraldehyde coupling. Binding between the target odorant, amyl butyrate, and the OR2AG1 receptor protein generated a change in resistance of the graphene resistor sensor. The functionalized graphene resistor sensors exhibited a linear sensor response between 0.1-500 pM and high selectively towards amyl butyrate, with a sensitivity as low as 500 fM, whilst control measurements using non-specific esters, produced a negligible sensor response. The approach described here provides an alternative sensing platform that can be used in bioelectronic nose applications.