A study of varicella zoster virus glycoprotein C regulatory region response to viral activators in vitro.
A study of varicella zoster virus glycoprotein C regulatory region response to viral activators in vitro.
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DOI：
10.3923/pjbs.2007.2140.2145
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发表时间：
2007-07-01
期刊：
影响因子：
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通讯作者：
Sabahi, Farzaneh
中科院分区：
文献类型：
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作者：
Gharavi, Sara;Sadeghizadeh, Majid;Sabahi, Farzaneh
进行了一项研究，分析水痘带状疱疹病毒（VZV）糖蛋白C基因（ORF 14）转录位点下游和上游调节序列对VZV反式激活因子IE 4和IE 62以及p29（单链DNA结合蛋白）的反应，通过体外瞬时转染允许的人黑色素瘤细胞系（Mewo）。这种糖蛋白已被证明是VZV发病机制中的一个重要因素，因此其表达的调控一直备受关注。在这项研究中，gC的启动子区以及另一个VZV糖蛋白，gI（作为阳性对照），扩增和克隆到启动子少质粒表达荧光素酶基因作为报告。通过定量荧光素酶活性来评估来自两种糖蛋白的调节区的活性。结果表明，荧光素酶试验是测量启动子活性的有力手段;然而，真正晚期gC基因的启动子区和同源下游和上游序列对这些病毒蛋白没有反应，表明其他病毒/细胞因子和/或病毒复制可能参与VZV感染周期期间的gC合成。
A study was conducted to analyze the response of varicella zoster virus (VZV) glycoprotein C gene (ORF14) regulatory sequences downstream as well as upstream of the transcription site to VZV transactivators, IE4 and IE62 and p29, the single-stranded DNA binding protein, in vitro by transiently transfecting a permissive human melanoma cell line (Mewo). This glycoprotein has been shown to be an important factor in VZV pathogenesis and therefore the regulation of its expression has been of much interest. In this study, the promoter region of gC as well as another VZV glycoprotein, gI (as a positive control), was amplified and cloned into a promoter less plasmid expressing the luciferase gene as a reporter. The activities of the regulatory regions from both glycoproteins were assessed by quantifying the luciferase activity. The results show that the luciferase assay is a powerful means of measuring promoter activity; nevertheless, the promoter region and cognate downstream and upstream sequences of the true late gC gene were not responsive to these viral proteins, indicating that other viral/cellular factors and/or viral replication could be involved in gC synthesis during the VZV infection cycle.