Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during Drosophila development
Dacapo, a cyclin-dependent kinase inhibitor, stops cell proliferation during Drosophila development
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DOI：
10.1016/s0092-8674(00)81818-8
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发表时间：
1996-12-27
期刊：
影响因子：
64.5
通讯作者：
Vaessin, H
中科院分区：
文献类型：
--
作者：
Lane, ME;Sauer, K;Vaessin, H
在多细胞真核生物中，大多数细胞类型在终分化前退出有丝分裂细胞周期。我们发现，在果蝇胚胎发生的正确发育阶段，需要dacapo基因来阻止表皮细胞的增殖。dacapo编码细胞周期蛋白E/cdk2复合物的抑制剂，与脊椎动物Cip/Kip抑制剂相似。dacapo在有丝分裂前的G2期晚期开始短暂表达。不能表达该抑制剂的突变体不能在有丝分裂16后阻止细胞增殖，并通过一个额外的分裂周期进行。相反，转基因胚胎中过早的dacapo表达会导致早熟G1阻滞。
Most cell types in multicellular eukaryotes exit from the mitotic cell cycle before terminal differentiation. We show that the dacapo gene is required to arrest the epidermal cell proliferation at the correct developmental stage during Drosophila embryogenesis. dacapo encodes an inhibitor of cyclin E/cdk2 complexes with similarity to the vertebrate Cip/Kip inhibitors. dacapo is transiently expressed beginning late in the G2 phase preceding the terminal division (mitosis 16). Mutants unable to express the inhibitor fail to arrest cell proliferation after mitosis 16 and progress through an extra division cycle. Conversely, premature dacapo expression in transgenic embryos results in a precocious G1 arrest.