No evidence of association between the synonymous polymorphisms in XRCC1 and ERCC2 and breast cancer susceptibility among nonsmoking Chinese.
No evidence of association between the synonymous polymorphisms in XRCC1 and ERCC2 and breast cancer susceptibility among nonsmoking Chinese.
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DOI：
10.1016/j.gene.2012.04.072
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发表时间：
2012-07
期刊：
影响因子：
3.5
通讯作者：
Jiaoyang Yin;Chunhong Wang;D. Liang;U. Vogel;L. Yue;Jian Liu;R. Qi;Xiaoling Sun
中科院分区：
文献类型：
--
作者：
Jiaoyang Yin;Chunhong Wang;D. Liang;U. Vogel;L. Yue;Jian Liu;R. Qi;Xiaoling Sun
DNA 修复能力也被认为是乳腺癌的潜在易感因素。 DNA 修复基因的同义多态性与乳腺癌相关的作用仍然很大程度上未知。非吸烟者是研究癌症遗传易感性的良好模型，因为他们接触的致癌物剂量较低。为了验证该疾病的遗传生物标志物，我们探讨了染色体 19q13.2-3 上 DNA 修复基因 XRCC1 和 ERCC2 的两个同义多态性 [Pro206Pro (rs915927) 和 Arg156Arg (rs238406)] 对不吸烟的中国人乳腺癌易感性的影响。该研究招募了 243 名乳腺癌患者和 234 名无癌症对照者，这些患者的年龄（±3 岁）、性别、不吸烟状况和种族与病例相匹配。使用聚合酶链反应-限制性片段长度多态性确定基因型。在个体单核苷酸多态性或单倍型与乳腺癌易感性之间没有观察到关联。分层后，没有检测到与乳腺癌发生相关的年龄依赖性效应或绝经状态。没有证据表明基因与基因之间的相互作用影响乳腺癌易感性。两个位点处于弱连锁不平衡状态（D′值=0.244，P=0.07）。目前的数据表明，XRCC1 Pro206Pro 和 ERCC2 Arg156Arg 对不吸烟的中国人的乳腺癌易感性没有显着影响。
DNA repair proficiency has also been proposed as a potential susceptibility factor for breast cancer. Synonymous polymorphism roles of the DNA repair genes in relation to breast cancer remain largely unknown. Nonsmokers are a good model in which to investigate genetic susceptibility to cancer because they are at low-dose carcinogen exposure. To validate genetic biomarkers of the disease, we explored the effects of the two synonymous polymorphisms [Pro206Pro (rs915927) and Arg156Arg (rs238406)] in the DNA repair genes XRCC1 and ERCC2 at chromosome 19q13.2–3 on breast cancer susceptibility among nonsmoking Chinese. The study recruited 243 patients with breast cancer and 234 cancer-free controls matched to the cases by age (±3years), gender, nonsmoking status and ethnicity. Genotypes were determined using polymerase chain reaction–restriction fragment length polymorphism. No associations were observed between both individual single nucleotide polymorphisms or haplotypes and breast cancer susceptibility. After stratification, no effects were detected for age-dependent effects or menopause status in relation to breast cancer occurrence. No evidence of gene–gene interaction in breast cancer susceptibility was revealed. The two loci were at weak linkage disequilibrium (D′ value=0.244, P=0.07). The present data suggest that XRCC1 Pro206Pro and ERCC2 Arg156Arg do not substantially influence breast cancer susceptibility among nonsmoking Chinese.