Porphyromonas gingivalis strain-dependent inhibition of uterine spiral artery remodeling in the pregnant rat.
Porphyromonas gingivalis strain-dependent inhibition of uterine spiral artery remodeling in the pregnant rat.
复制标题
牙龈卟啉单胞菌菌株依赖性抑制妊娠大鼠子宫螺旋动脉重塑。
DOI：
10.1093/biolre/ioy119
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发表时间：
2018
影响因子：
3.6
通讯作者：
Reyes,Leticia
中科院分区：
文献类型：
--
作者：
Phillips,Priscilla;Brown,MaryB;Progulske-Fox,Ann;Wu,Xiao-Jun;Reyes,Leticia
牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）是一种重要的牙周致病菌，也与妊娠并发症有关，包括有缺陷的深胎盘形成（defective deep placement，DDP）。我们推测PG侵入胎盘床促进DDP。妊娠大鼠在妊娠第14天（GD）静脉接种无菌溶剂、Pg菌株W83或A7436（急性队列）。非妊娠大鼠在繁殖前接受3个月的重复经口接种（慢性队列）。在GD 18收集组织和/或血清用于分析。通过血清转化（慢性队列）和子宫胎盘组织中是否存在Pgantigen来确定Pg感染状态，所述子宫胎盘组织用于螺旋动脉重塑的组织学和形态学评估。分析来自血清阳性母鼠的子宫系膜组织的白细胞介素1β、6和10、TNF、TGF-β、卵泡抑素相关蛋白3和卵泡抑素β A链的表达，因为这些基因调节绒毛外滋养层侵袭。两个队列中子宫胎盘组织中W83和A7436抗原的原位分布相似。急性期W83感染组子宫系膜间质坏死较常见，而A7436感染组动脉炎较常见（P< 0.05）。慢性感染组子宫系膜血管坏死明显增多（P< 0.05）。只有A7436感染的动物胎仔死亡率增加，螺旋动脉重塑减少，Escherichin β A表达减少，螺旋动脉内FSLT 3阳性绒毛外滋养层细胞比例增加。虽然感染两种Pg菌株产生不同的病理学的胎盘床，只有感染菌株A7436导致受损的螺旋动脉重塑。
Porphyromonas gingivalis(Pg) is an important periodontal pathogen that is also implicated in pregnancy complications involving defective deep placentation (DDP). We hypothesized thatPginvasion of the placental bed promotes DDP. Pregnant rats were intravenously inoculated with sterile vehicle,Pgstrain W83, or A7436 at gestation day (GD) 14 (acute cohort). Nonpregnant rats received repeated oral inoculations for 3 months before breeding (chronic cohort). Tissues and/or sera were collected at GD18 for analysis.Pginfection status was determined by seroconversion (chronic cohort) and by presence ofPgantigen in utero-placental tissues processed for histology and morphometric assessment of spiral artery remodeling. Mesometrial tissues from seropositive dams were analyzed for expression of interleukin 1β, 6, and 10, TNF, TGF-β, follistatin-related protein 3, and inhibin beta A chain since these genes regulate extravillous trophoblast invasion. The in situ distribution of W83 and A7436 antigen in utero-placental tissues was similar in both cohorts. In the acute cohort, mesometrial stromal necrosis was more common with W83, but arteritis was more common with A7436 infection (P< 0.05). Increased vascular necrosis was seen in mesometrium of chronically infected groups (P< 0.05). Only A7436-infected animals had increased fetal deaths, reduced spiral artery remodeling, reduced inhibin beta A expression, and an increased proportion of FSLT3 positive extravillous trophoblasts within spiral arteries. While infection with both Pg strains produced varying pathology of the deep placental bed, only infection with strain A7436 resulted in impaired spiral artery remodeling.