Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm.
Antibacterial TAP-mimic electrospun polymer scaffold: effects on P. gingivalis-infected dentin biofilm.
复制标题
抗菌TAP-MIMIC电纺聚合物支架：对牙龈疟原虫感染的牙本质生物膜的影响。
DOI：
10.1007/s00784-015-1577-2
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发表时间：
2016-03
影响因子：
3.4
通讯作者：
Bottino MC
中科院分区：
文献类型：
--
作者：
Albuquerque MT;Evans JD;Gregory RL;Valera MC;Bottino MC
本研究试图在体外研究最近开发的三抗生素糊体（TAP）模拟聚合物纳米纤维支架对牙龈卟啉单胞菌（Pg）感染的牙本质生物膜的作用。牙本质标本（4×4×1mm3）由人犬制备。对标本进行灭菌，接种Pg (ATCC 33277)，孵育一周，使生物膜形成。感染的牙本质标本暴露于以下处理3天：无抗生素聚二氧环酮支架（PDS，对照），PDS+25wt。%TAP（每毫升PDS聚合物溶液中每种抗生素[甲硝唑、环丙沙星和米诺环素]25毫克），或饱和TAP基溶液（每毫升生理盐水溶液中每种抗生素50毫克）。作为阴性对照，感染的牙本质标本未经处理（仅细菌）。为了确定tap模拟支架的抗菌效果，进行了集落形成单位（CFU/mL） （n=10/组）的测量。此外，制备额外的样品（n=2/组），通过扫描电子显微镜（SEM）定性研究生物膜的抑制作用。进行统计学处理，显著性设为5%水平。TAP模拟支架和阳性对照（TAP溶液）均能完全消除细菌，与阴性对照组（仅细菌）差异有统计学意义（p<0.05）。无抗生素支架组（2.7 log10 CFU/mL）与阴性对照组（5.9 log10 CFU/mL） CFU/mL数据无统计学差异。获得的数据显示，新型基于pds的tap模拟支架对已建立的pg感染的牙本质生物膜具有显着的抗菌性能。总的来说，这些数据表明，所提出的纳米纤维支架可能被用作替代临床提倡的金标准（即TAP），用于再生牙髓治疗前的管内消毒。
This study sought to investigate, in-vitro, the effects of a recently developed triple antibiotic paste (TAP)-mimic polymer nanofibrous scaffold against Porphyromonas gingivalis (Pg)-infected dentin biofilm. Dentin specimens (4×4×1mm3) were prepared from human canines. The specimens were sterilized, inoculated with Pg (ATCC 33277), and incubated for one week to allow for biofilm formation. Infected dentin specimens were exposed for 3 days to the following treatments: antibiotic-free polydioxanone scaffold (PDS, control), PDS+25wt.%TAP (25 mg of each antibiotic [metronidazole, ciprofloxacin, and minocycline] per mL of the PDS polymer solution), or a saturated TAP-based solution (50 mg of each antibiotic per mL of saline solution). In order to serve as the negative control, infected dentin specimens were left untreated (bacteria only). To determine the antimicrobial efficacy of the TAP-mimic scaffold, a colony-forming unit (CFU/mL) (n=10/group) measurement was performed. Furthermore, additional specimens (n=2/group) were prepared to qualitatively study biofilm inhibition via scanning electron microscopy (SEM). Statistics were performed and significance was set at the 5% level. Both the TAP-mimic scaffold and the positive control (TAP solution) led to complete bacterial elimination, differing statistically (p<0.05) from the negative control group (bacteria only). No statistical differences were observed for CFU/mL data between antibiotic-free scaffolds (2.7 log10 CFU/mL) and the negative control (5.9 log10 CFU/mL). The obtained data revealed significant antimicrobial properties of the novel PDS-based TAP-mimic scaffold against an established Pg-infected dentin biofilm. Collectively, the data suggest that the proposed nanofibrous scaffold might be used as an alternative to the advocated clinical gold standard (i.e., TAP) for intracanal disinfection prior to regenerative endodontics.