Glial retinal Müller cells produce IL-1 activity and have a dual effect on autoimmune T helper lymphocytes. Antigen presentation manifested after removal of suppressive activity.
Glial retinal Müller cells produce IL-1 activity and have a dual effect on autoimmune T helper lymphocytes. Antigen presentation manifested after removal of suppressive activity.
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DOI：
10.4049/jimmunol.140.7.2193
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发表时间：
1988-04
影响因子：
4.4
通讯作者：
F. Roberge;R. Caspi;R. Nussenblatt
中科院分区：
文献类型：
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作者：
F. Roberge;R. Caspi;R. Nussenblatt
Müller细胞是哺乳动物视网膜的主要胶质细胞。当暴露于活化淋巴细胞的上清液时，可以诱导其在培养物中表达MHC II类决定簇。在体外共培养系统中，Müller细胞已被证明对Ag和IL-2驱动的Th细胞系增殖产生深刻的抑制影响。在本报告中，我们证明了Müller细胞可以产生IL-1活性，并且在其抑制作用被抑制的条件下，它们显示出有效发挥APC功能的能力。用含Ag和IFN-γ的大鼠脾条件培养液预孵育轻度胰蛋白酶化和戊二醛固定的Müller细胞，能够将Ag呈递给S-Ag特异性Th淋巴细胞系，该细胞系具有诱导大鼠严重葡萄膜视网膜炎的能力。固定后添加Ag不会导致呈递，表明Müller细胞必须将标称Ag加工成免疫原性形式。还发现，在幼稚同基因或同种异体辅助细胞的存在下，呈递效率大大增加。这种增强作用不能用rIL-1复制。本报告扩展了我们正在进行的视网膜神经胶质Müller细胞和T淋巴细胞之间的相互作用的研究。结果表明，这些相互作用可以是多样的，甚至在不同的条件下是相反的，并表明Müller细胞可能在神经视网膜水平的免疫反应过程中发挥决定性作用。
The Müller cell is the major glial element of the mammalian retina. It can be induced to express MHC class II determinants in culture, when exposed to supernatant from activated lymphocytes. In an in vitro co-culture system, Müller cells had been shown to exert a profound inhibitory influence on Ag- and IL-2-driven proliferation of Th cell lines. In the present report we demonstrate that Müller cells can produce IL-1 activity, and that in conditions where their inhibitory action is suppressed, they display the capacity to efficiently function as APC. Mildly trypsinized and glutaraldehyde-fixed Müller cells that had been preincubated with Ag and IFN-gamma-containing rat spleen conditioned medium were able to present Ag to an S-Ag specific Th lymphocyte line, which has the capacity to induce a severe uveoretinitis in rats. Addition of the Ag after fixation did not result in presentation, showing that Müller cells had to process the nominal Ag into an immunogenic form. It was also found that the efficiency of presentation was greatly increased in the presence of naive syngeneic or allogeneic accessory cells. This enhancing effect could not be reproduced with rIL-1. This report extends our ongoing study of interactions between the retinal glial Müller cell and T lymphocytes. The results show that these interactions can be diverse, even opposite in different conditions, and suggest the possibility that Müller cells could play a determining role in the course of immune reactions at the level of the neuro-retina.