Electrostatic Cell-Surface Repulsion Initiates Lumen Formation in Developing Blood Vessels
Electrostatic Cell-Surface Repulsion Initiates Lumen Formation in Developing Blood Vessels
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DOI：
10.1016/j.cub.2010.09.061
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发表时间：
2010-11-23
期刊：
影响因子：
9.2
通讯作者：
Lammert, Eckhard
中科院分区：
文献类型：
--
作者：
Strilic, Boris;Eglinger, Jan;Lammert, Eckhard
Blood vessels function in the uptake, transport, and delivery of gases and nutrients within the body A key question is how the central lumen of blood vessels develops within a cord of vascular endothelial cells Here, we demonstrate that maim acids of apical glycoproteins localize to apposing endothelial cell surfaces and generate repelling electrostatic fields within an endothelial cell cord Both in vitro and in vivo experiments show that the negative charge of maim acids is required for the separation of endothelial cell surfaces and subsequent lumen formation We also demonstrate that sulfate residues can substitute for sialic acids during lumen initiation These results therefore reveal a key step in the creation of blood vessels, the most abundant conduits in the vertebrate body Because negatively charged mucins and proteoglycans are often found on luminal cell surfaces, It is possible that electrostatic repulsion is a general principle also used to initiate lumen formation in other organs
