Intragastric intubation: Important aspects of the model for administration of ethanol to rat pups during the postnatal period
Intragastric intubation: Important aspects of the model for administration of ethanol to rat pups during the postnatal period
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DOI：
10.1097/00000374-199810000-00032
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发表时间：
1998-10-01
影响因子：
3.2
通讯作者：
Nyamweya, N
中科院分区：
文献类型：
--
作者：
Light, KE;Kane, CJM;Nyamweya, N
一种用于向新生大鼠幼崽控制递送乙醇的技术是胃内插管。通常，用于递送乙醇的媒介物由营养混合物组成，以补偿减少的哺乳或其他可能的营养损害。本研究分析了营养载体的选择、一窝内实验处理组的组合以及总体窝仔数对乙醇插管和插管对照幼仔生长速率的影响，并与母亲饲养的对照幼仔进行了比较。将Sprague-Dawley大鼠幼仔以8或10窝一窝饲养，并通过胃内插管给予乙醇和20%（v/v）Sustacal（R）或80%（v/v）Intraperid-II（R）营养载体。幼仔在出生后第2天至第10天之间给药，并在第10天分析体重。幼崽被分配到一个治疗组，作为插管乙醇，插管对照，或非插管母亲提出的控制。通过统计分析进行实验比较，以确定最佳处理设计（单窝混合处理组或每窝单处理组）、最佳溶剂（Sustacal（R）或Intradecid-II（R））和每窝幼仔的最佳数量（8 vs. 10）。分析表明，在一窝中混合插管对照组、插管乙醇组和未插管母鼠饲养的对照治疗组引入了一个非受控变量，该变量混淆了乙醇特异性变化的测量。处理组对纳入混合窝仔的敏感性取决于营养溶剂酸，因此取决于营养充足性。我们的研究结果表明，最佳的设计，实现了每窝8只小狗。此外，当使用Intralid-II（R）作为营养媒介物时，乙醇插管和插管对照幼仔的生长速率与8只母亲饲养的对照幼仔的平行窝相同，并且在一窝中存在单个处理组。这些实验参数的优化提供了一个很好的新生大鼠模型，用于分析特定的乙醇对大脑发育的影响在孕晚期。
One technique for the controlled delivery of ethanol to neonatal rat pups is intragastric intubation. Often, the vehicle used for delivery of ethanol is composed of a nutrient mixture to compensate for decreased suckling or other possible nutritional compromise. This study analyzed the selection of nutrient vehicle, the combination of experimental treatment groups within a litter, and the overall litter size on the growth rate of ethanol-intubated and intubated-control pups, compared with mother-raised control pups. Sprague-Dawley rat pups were raised in litters of 8 or 10, and administered ethanol by intragastric intubation with 20% (v/v) Sustacal(R) or 80% (v/v) Intralipid-II(R) nutrient vehicle. Pups were treated between postnatal days 2 and 10, and body weight was analyzed on day 10. Pups were assigned to a treatment group as either intubated ethanol, intubated control, or nonintubated mother-raised controls. Experimental comparison by statistical analyses was performed to identify the optimal treatment design (mixed treat ment groups in a single litter or a single treatment group per litter), the optimal vehicle (Sustacal(R) or Intralipid-II(R)), and the optimal number of pups per litter (8 vs. 10). The analyses demonstrate that the mixing of intubated control, intubated ethanol, and nonintubated mother-raised control treatment groups within a single litter introduced an uncontrolled variable that confounded measurement of ethanol-specific alterations. The sensitivity of treatment groups to inclusion in mixed litters was dependent on the nutrient vehicle acid thus nutritional adequacy. Our results suggest that an optimal design was achieved with eight pups per litter. Furthermore, ethanol intubated and intubated control pups grow at a rate Identical to parallel litters of eight mother-raised control pups when Intralipid-II(R) is used as nutrient vehicle, and a single treatment group is present in a litter. Optimization of these experimental parameters has provided an excellent neonatal rat model for analysis of specific ethanol effects on brain development during the third trimester.