Drosophila starvin encodes a tissue-specific BAG-domain protein required for larval food uptake
Drosophila starvin encodes a tissue-specific BAG-domain protein required for larval food uptake
复制标题
DOI：
10.1534/genetics.105.043265
复制
发表时间：
2005-12-01
期刊：
影响因子：
3.3
通讯作者：
Saint, R
中科院分区：
文献类型：
--
作者：
Coulson, M;Robert, S;Saint, R
我们描述了BAG基因家族中唯一的果蝇成员的发育、遗传和分子分析，该基因与哺乳动物细胞的应激反应和存活有关。我们发现，这种被称为stv的基因以一种高度组织特异性的方式表达，主要在胚带收缩后的肌腱细胞中积累，随后在胚胎15期的躯体肌肉和食道中积累。我们发现stv表达在已知的肌腱和肌肉细胞转录调节级联中，位于stripe的下游，但不位于另一种肌腱转录调节因子delilah和肌肉调节因子mef-2的下游。我们产生了一系列stv等位基因，令人惊讶的是，考虑到stv的肌肉和肌腱特异性胚胎表达，我们发现stv突变体的体细胞肌肉的大体形态和功能是正常的。尽管如此，stv突变体幼虫表现出一种惊人的、完全渗透的突变表型，即孵化后不能生长，并且严重损害了吸收食物的能力。我们的研究首次报道了一个重要的、受发育调节的bag家族基因。
We describe a developmental, genetic, and molecular analysis of the sole Drosophila member of the BAG family of genes, which is implicated in stress response and survival in mammalian cells. We show that the gene, termed starvin (stv), is expressed in a highly tissue-specific manner, accumulating primarily in tendon cells following germ-band retraction and later in somatic muscles and the esophagus during embryonic stage 15. We show that stv expression falls within known tendon and muscle cell transcriptional regulatory cascades, being downstream of stripe, but not of another tendon transcriptional regulator, delilah, and downstream of the muscle regulator, mef-2. We generated a series of stv alleles and, surprisingly, given the muscle and tendon-specific embryonic expression of stv, found that the gross morphology and function of somatic muscles is normal in stv mutants. Nonetheless, stv mutant larvae exhibit a striking and fully penetrant mutant phenotype of failure to grow after hatching and a severely impaired ability to take up food. Our study provides the first report of an essential, developmentally regulated BAG-family gene.