Strontium-substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration
Strontium-substituted biphasic calcium phosphate microspheres promoted degradation performance and enhanced bone regeneration
复制标题
锶取代的双相磷酸钙微球促进降解性能并增强骨再生
DOI：
10.1002/jbm.a.36867
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发表时间：
2019-12-26
影响因子：
4.9
通讯作者：
Li, Bo
中科院分区：
文献类型：
--
作者：
Chen, Ying;Liu, Zhongning;Li, Bo
双相磷酸钙（BCP）陶瓷作为骨替代材料受到广泛关注。然而，促进BCP陶瓷的降解和骨诱导性能仍然是一个挑战。本工作通过高锶掺杂制备了具有良好降解性和良好骨诱导性的新型BCP陶瓷微球。体外实验结果表明，Sr 10-BCP、Sr 40-BCP和Sr 80-BCP微球在高温烧结后，HA晶体部分转变为β-磷酸三钙相，这是由于Sr离子部分取代Ca离子而形成缺钙HA。此外，随着Sr掺杂量的增加，降解速率增加。所有制备的微球增强人骨髓间充质干细胞的附着和增殖。具体而言，在这些改性微球中，Sr 40-BCP微球显示出最高的成骨潜力。将Sr 40-BCP和HA微球植入大鼠颅骨缺损模型，评价其体内骨增量能力。结果表明，与HA微球相比，Sr 40-BCP微球降解更彻底，能显著促进新骨再生。总之，Sr 40-BCP微球具有良好的降解和骨再生潜力，是一种有前途的成骨材料。
Biphasic calcium phosphate (BCP) ceramics are the subject of much attention for use as bone replacement material. However, it remains a challenge to promote the degradation and osteoinductivity performances of BCP ceramics. In this work, novel BCP ceramic microspheres with good degradation and excellent osteoinductivity were prepared through high-content strontium (Sr) doping. The in vitro results indicated that the Sr10-BCP, Sr40-BCP, and Sr80-BCP microspheres all had their HA crystals partially transformed to the beta tricalcium phosphate phase following high-temperature sintering because of Ca-deficient HA formed by the partial substitution of Ca ions by Sr ions. In addition, the degradation rate was increased with the doping of increasing amounts of Sr. All prepared microspheres enhanced human bone marrow-derived mesenchymal stem cells attachment and proliferation. Specifically, among these modified microspheres, the Sr40-BCP microspheres showed the highest osteogenic potential. Furthermore, Sr40-BCP and HA microspheres were implanted in a calvarial defect model of rat to evaluate the in vivo bone augmentation ability. The results indicated that Sr40-BCP microspheres degraded more completely and significantly promoted new bone regeneration compared with HA microspheres. In conclusion, Sr40-BCP microspheres have excellent potential for degradation and bone regeneration and are promising osteogenic materials.