Myostatin promoter analysis and expression pattern in pigs
Myostatin promoter analysis and expression pattern in pigs
复制标题
DOI：
10.1016/j.livsci.2007.02.004
复制
发表时间：
2007-10-01
影响因子：
1.8
通讯作者：
Rothschild, Max F.
中科院分区：
文献类型：
--
作者：
Guimaraes, Simone E. F.;Stahl, Chad H.;Rothschild, Max F.
肌肉生长抑制素是转化生长因子超家族的成员，在出生前和出生后的骨骼肌中表达。肌肉生长抑制素可防止肌肉发育过程中成肌细胞的过度分化。肌生长抑制素功能缺失突变会产生多余的肌纤维，导致肌肉增生。尽管肌生长抑制素突变对不同的牛品种有很大影响，但尚未描述对猪产生重大影响的突变。在这项研究中，我们鉴定了肌肉生长抑制素基因启动子区域的单核苷酸多态性 (SNP)，研究了等位基因频率，分析了背最长肌中的基因表达模式，并对猪的生产性能、胴体成分和肉品质性状进行了统计关联。这些分析中包括了来自不同品种和商业品系的猪。鉴定出两个处于完全连锁不平衡的 SNP，G-847A 和 A-835G。对他们的等位基因和基因型频率进行了分析并进行了关联研究。通过半定量实时 PCR 测定肌肉生长抑制素基因表达，并与表型性状相关。 SNP 相关（P
Myostatin, a member of the transforming growth factor superfamily, is expressed in skeletal muscle both pre- and postnatally. Myostatin prevents excessive differentiation of myoblasts during muscular development. Loss of function mutations to Myostatin can produce supernumerary muscle fibers, resulting in muscle hyperplasia. Despite the large effects of Myostatin mutations in different cattle breeds, no mutations with major effects have been described in pigs. In this study, we identified single nucleotide polymorphisms (SNPs) in the promoter region of the Myostatin gene, investigated allele frequencies, analyzed gene expression pattern in the longissimus dorsi muscle and performed statistical associations with performance, carcass composition and meat quality traits in pigs. Pigs from different breeds and commercial lines were included in these analysis. Two SNPs, G-847A and A-835G, in complete linkage disequilibrium were identified. Their allele and genotypic frequencies were analyzed and an association study conducted. Determination of Myostatin gene expression was obtained by semi-quantitative real-time PCR and correlated to phenotypic traits. The SNPs were associated (P