Sciatic nerve regeneration through venous or nervous grafts in the rat
Sciatic nerve regeneration through venous or nervous grafts in the rat
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DOI：
10.1006/exnr.1997.6542
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发表时间：
1997-11-01
影响因子：
5.3
通讯作者：
Lejeune, G
中科院分区：
文献类型：
--
作者：
FoidartDessalle, M;Dubuisson, A;Lejeune, G
本研究分析了35只Wistar大鼠用生理盐水或雪旺细胞填充的坐骨静脉移植物与神经移植物作为坐骨神经再生的指导的兴趣。术后1个月至1年，多次测量电生理参数（传导速度和运动反应的远端潜伏期）和De Medinacelli功能指数。对2只对照大鼠和3只手术后6个月或12个月处死的大鼠进行组织学分析：在整个神经的蒙太奇图像上计数纤维总数，并在数字化图像上测量轴突直径和髓鞘厚度。用一部分神经作为引导，再生比静脉快。然而，6个月后的再生至少与填充有雪旺细胞的静脉移植物一样好，如通过电生理学、功能和组织学分析所评估的。在移植静脉中添加施万细胞允许神经通过比先前描述的更长的间隙（25 mm vs 15 mm）再生。为了评估神经再生的质量，功能、电生理和组织学分析是互补的。(C)北京：科学出版社.
This study analyses the interest of isologous venous grafts filled with saline or with Schwann cells versus nerve grafts as guides for regeneration of the sciatic nerve in 35 Wistar rats, Electrophysiological parameters (conduction velocities and distal latencies of motor responses) and the functional index of De Medinacelli were measured several times from 1 month to 1 year after surgery. An histological analysis was performed on 2 control rats and on 3 rats killed 6 or 12 months after surgery: the total number of fibers was counted on a montage photoprint of the whole nerve, and the diameters of axons and the thickness of the myelin sheath were measured on digitized images. With a portion of nerve as guide, the regeneration is faster than with a vein. However, regeneration after 6 months is at least as good with a venous graft filled with Schwann cells, as assessed by electrophysiological, functional, and histological analysis. The addition of Schwann cells in grafted veins allows the nerve to regenerate through longer gaps than previously described (25 vs 15 mm). In order to assess the quality of nerve regeneration, functional, electrophysiological, and histological analysis are complementary. (C) 1997 Academic Press.