Mitogenic activity of rat salivary glands after electrical stimulation of parasympathetic nerves.
Mitogenic activity of rat salivary glands after electrical stimulation of parasympathetic nerves.
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电刺激副交感神经后大鼠唾液腺的有丝分裂活性。
DOI：
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发表时间：
1993
影响因子：
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通讯作者：
D. Jirakulsomchok
中科院分区：
文献类型：
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作者：
C. Schneyer;M. Humphreys;H. Hall;D. Jirakulsomchok
18小时后进行测量时，对腮腺或下颌下腺的副交感神经支配进行30或60分钟的电刺激会导致两个腺体的[3H]胸苷摄取增加。刺激30分钟后，与未受刺激的伴侣相比，腮腺平均增加30%，刺激60分钟后，增加76%。用存在的肾上腺素能拮抗剂普萘洛尔和苯氧基苯扎明刺激 30 分钟，显示[3H]胸苷增加了 76%。与未刺激的交感神经切除的伴侣相比，刺激鼓索 30 分钟导致交感神经切除的颌下腺的胸苷摄取平均增加 59%。受刺激的腮腺和颌下腺的 β-1,4-半乳糖基转移酶活性也增加了 71%。根据口腔唾液的成分，我们确认腮腺受刺激的神经为副交感神经（耳颞神经）。 Na、K 和淀粉酶浓度与通过电刺激副交感神经直接从导管获得的唾液成分非常相似。这些数据提供了第一个证据，证明副交感神经对腮腺和颌下腺的电刺激会在这些器官中引起有丝分裂反应；这些数据还提供了第一个证据，表明副交感神经的电刺激会导致β-1,4-半乳糖基转移酶水平升高，已知这种酶与增生反应有关。
Electrical stimulation of the parasympathetic innervation to parotid or submandibular gland for 30 or 60 min resulted in increased [3H]thymidine uptake of both glands when measurements were made 18 h later. With 30 min of stimulation, the mean increase in parotid was 30% compared with unstimulated mates, and after 60 min of stimulation, the increase was 76%. Stimulation for 30 min with the adrenergic antagonists propranolol and phenoxybenzamine present showed an increase in [3H]thymidine of 76%. Stimulation of the chorda tympani for 30 min resulted in a mean increase of 59% in thymidine uptake of the sympathectomized submandibular gland compared with the unstimulated sympathectomized mate. beta-1,4-Galactosyltransferase activity of both stimulated parotid and submandibular glands also showed an increase of 71%. On the basis of the composition of the saliva in the oral cavity, we confirmed the identity of the stimulated nerve to parotid gland as the parasympathetic (auriculotemporal). Na, K, and amylase concentrations resembled closely the composition of saliva obtained directly from the duct with electrical stimulation of the parasympathetic nerve. These data provide the first evidence that electrical stimulation of the parasympathetic nerve to parotid and submandibular glands causes a mitogenic response in each of these organs; the data also provide the first evidence that electrical stimulation of the parasympathetic nerve causes an increase in levels of the enzyme beta-1,4-galactosyltransferase, known to be implicated in hyperplastic responses.