Insulin Signaling Plays a Dual Role in Caenorhabditis elegans Memory Acquisition and Memory Retrieval
Insulin Signaling Plays a Dual Role in Caenorhabditis elegans Memory Acquisition and Memory Retrieval
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DOI：
10.1523/jneurosci.4636-09.2010
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发表时间：
2010-06-09
影响因子：
5.3
通讯作者：
van der Kooy, Derek
中科院分区：
文献类型：
--
作者：
Lin, Chia Hsun Anthony;Tomioka, Masahiro;van der Kooy, Derek
胰岛素信号在秀丽隐杆线虫的水形成和寿命调控中起着重要作用。在这里，我们发现胰岛素信号在苯甲醛饥饿联想可塑性中也是必需的，其中蠕虫在没有食物的情况下预先暴露于气味引诱剂苯甲醛，随后表现出对气味的条件厌恶反应。分别编码人类胰岛素、胰岛素/IGF-1受体和PIP3激酶同源基因的胰岛素相关1 （ins-1）、异常水形成2 （daf-2）和衰老改变1 （age-1）发生突变的动物，表现出苯甲醛饥饿相关可塑性的显著缺陷。使用一个条件等位基因，我们发现DAF-2信号在联想可塑性中的行为作用是可以分离的，DAF-2信号在记忆检索中比在记忆获取中发挥更重要的作用。我们提出DAF-2信号在苯甲醛-饥饿联想可塑性的记忆获取阶段作为学习特异性的饥饿信号，但在记忆检索过程中将苯甲醛感知的两栖动物翅膀C神经元转换为回避信号模式。
Insulin signaling plays a prominent role in regulation of dauer formation and longevity in Caenorhabditis elegans. Here, we show that insulin signaling also is required in benzaldehyde-starvation associative plasticity, in which worms pre-exposed to the odor attractant benzaldehyde in the absence of food subsequently demonstrate a conditioned aversion response toward the odorant. Animals with mutations in insulin-related 1 (ins-1), abnormal dauer formation 2 (daf-2), and aging alteration 1 (age-1), which encode the homolog of human insulin, insulin/IGF-1 receptor, and PIP3 kinase, respectively, demonstrated significant deficits in benzaldehyde-starvation associative plasticity. Using a conditional allele, we show that the behavioral roles of DAF-2 signaling in associative plasticity can be dissociated, with DAF-2 signaling playing a more significant role in the memory retrieval than in memory acquisition. We propose DAF-2 signaling acts as a learning-specific starvation signal in the memory acquisition phase of benzaldehyde-starvation associative plasticity but functions to switch benzaldehyde-sensing amphid wing C neurons into an avoidance signaling mode during memory retrieval.