Prevalence of methicillin-resistant Staphylococcus aureus in meat
Prevalence of methicillin-resistant Staphylococcus aureus in meat
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DOI：
10.1016/j.ijfoodmicro.2008.12.007
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发表时间：
2009-08-31
影响因子：
5.4
通讯作者：
Heuvelink, A. E.
中科院分区：
文献类型：
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作者：
de Boer, E.;Zwartkruis-Nahuis, J. T. M.;Heuvelink, A. E.
近年来，从几种食用动物中分离出耐甲氧西林金黄色葡萄球菌（MRSA）的报道。在屠宰MRSA阳性动物期间。可能发生MRSA对屠体的污染，因此这些动物的肉可能受到污染。本研究的目的是评估零售业生肉样本中耐甲氧西林金黄色葡萄球菌的流行情况，样本包括零售业的生牛肉、猪肉、小牛肉、羊肉/羊肉、鸡肉、火鸡、家禽和野味。一种检测方法，包括一个两步富集在Mueller-Hinton肉汤+ 6.5%氯化钠和酚红甘露醇肉汤含有头孢唑肟和氨曲南，然后通过分离的MRSA ID琼脂（bioMerieux）进行了评估，随后应用于检测生肉样品中的MRSA。从2217份分析样本中分离出264株（11.9%）MRSA菌株。肉类中的分离率依次为牛肉（10.6%）、小牛肉（15.2%）、羊肉（6.2%）、猪肉（10.7%）、鸡肉（16.0%）、火鸡（35.3%）、家禽（3.4%）和野味（2.2%）。大多数（85%）的分离菌株属于SPA型的脉冲场凝胶电泳（PFGE）不可分型（NT）-MRSA，对应于多位点序列类型ST 398。最近在荷兰也从猪身上分离出一种类型。然而.这些菌株中有一小部分被发现是其他ST的，可能是人类起源的。需要进一步的研究来阐明MRSA在肉类和其他食物中的传播途径，并提供预防MRSA传播的工具。目前，在肉类中的高流行率并未显示出对MRSA向人类传播的显着贡献，应进一步阐明食物中存在MRSA对消费者健康的可能危害。(C)2008 Elsevier B. V.保留所有权利。
Recently the isolation of methicillin-resistant Staphylococcus aureus (MRSA) strains from several food-producing animals has been reported. During slaughtering of MRSA-positive animals. contamination of carcasses with MRSA may occur and consequently the meat of these animals may get contaminated. The aim of this study was to estimate the prevalence of MRSA in raw meat samples from the retail trade.Samples of raw beef, pork, veal, lamb/mutton, chicken, turkey, fowl and game were collected from the retail trade. A detection method including a two-step enrichment in Mueller-Hinton broth + 6.5% NaCl and phenol red mannitol broth containing ceftizoxime and aztreonam, followed by isolation on MRSA ID agar (bioMerieux) was evaluated and subsequently applied for the detection of MRSA in samples of raw meats. MRSA strains were isolated from 264 (11.9%) of 2217 samples analyzed. Isolation percentages for the meat species were: beef (10.6%), veal (15.2%), lamb and mutton (6.2%), pork (10.7%), chicken (16.0%), turkey (35.3%), fowl (3.4%) and game (2.2%). The majority (85%) of the isolated strains belonged to spa-types of pulsed-field gel electrophoresis (PFGE) non-typeable (NT)-MRSA, corresponding to the multilocus sequence type ST398. a type also recently isolated in the Netherlands from pigs. However. a smaller part of these strains were found to be of other ST's, possibly of human origin.Further studies are needed to elucidate transmission routes of MRSA in relation to meat and other foods and to provide the tools for preventing the spread of MRSA. At present the high prevalence of MRSA in meat has not been shown to contribute significantly to the dissemination of MRSA to humans and the possible health hazard for consumers of the presence of MRSA in foods should be further elucidated. (C) 2008 Elsevier B.V. All rights reserved.