Palmitoylation of CM2 is dispensable to influenza C virus replication
Palmitoylation of CM2 is dispensable to influenza C virus replication
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DOI：
10.1016/j.virusres.2011.02.013
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发表时间：
2011-04-01
期刊：
影响因子：
5
通讯作者：
Ohara, Yoshiro
中科院分区：
文献类型：
--
作者：
Muraki, Yasushi;Okuwa, Takako;Ohara, Yoshiro
CM2是丙型流感病毒的第二膜蛋白。尽管已经确定了修饰后的氨基酸在CM2上的位置，但在病毒复制的背景下，CM2翻译后修饰的意义仍有待澄清。在本研究中，我们利用反向遗传学技术，产生了一种缺乏CM2棕榈酰化位点的重组流感病毒rCM2-C65A，其中CM2残基65处的半胱氨酸突变为丙氨酸，并检测了病毒在重组感染细胞中的生长和病毒蛋白合成。rCM2-C65A病毒在培养的HMV-II细胞和有胚的鸡蛋中和亲本病毒一样有效地生长。在rcm2 - c65a感染的HMV-II细胞中HEF、NP、M1和突变体CM2的合成和生化特征与亲本病毒感染的细胞相似。此外，对感染细胞的膜浮选分析表明，rcm2 - c65a感染细胞的质膜组分中回收的病毒蛋白与亲本病毒感染细胞中的病毒蛋白相同。这些结果表明，CM2棕榈酰化缺陷不影响病毒感染细胞中HEF、NP、M1以及CM2的转运和成熟，CM2棕榈酰化对于C型流感病毒的复制是必不可少的。(C) 2011 Elsevier B.V.版权所有
CM2 is the second membrane protein of influenza C virus. The significance of the posttranslational modifications of CM2 remains to be clarified in the context of viral replication, although the positions of the modified amino acids on CM2 have been determined. In the present study, using reverse genetics we generated rCM2-C65A, a recombinant influenza C virus lacking CM2 palmitoylation site, in which cysteine at residue 65 of CM2 was mutated to alanine, and examined viral growth and viral protein synthesis in the recombinant-infected cells. The rCM2-C65A virus grew as efficiently as did the parental virus in cultured HMV-II cells as well as in embryonated chicken eggs. The synthesis and biochemical features of HEF, NP, M1 and mutant CM2 in the rCM2-C65A-infected HMV-II cells were similar to those in the parental virus-infected cells. Furthermore, membrane flotation analysis of the infected cells revealed that equal amount of viral proteins was recovered in the plasma membrane fractions of the rCM2-C65A-infected cells to that in the parental virus-infected cells. These findings indicate that defect in palmitoylation of CM2 does not affect transport and maturation of HEF, NP and M1 as well as CM2 in virus-infected cells, and palmitoylation of CM2 is dispensable to influenza C virus replication. (C) 2011 Elsevier B.V. All rights reserved.