THE MEASUREMENT OF LYSOZYME ACTIVITY AND THE ULTRA-VIOLET INACTIVATION OF LYSOZYME
THE MEASUREMENT OF LYSOZYME ACTIVITY AND THE ULTRA-VIOLET INACTIVATION OF LYSOZYME
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DOI：
10.1016/0006-3002(52)90045-0
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发表时间：
1952-01-01
期刊：
影响因子：
--
通讯作者：
SHUGAR, D
中科院分区：
文献类型：
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作者：
SHUGAR, D
美国v.溶菌酶的失活符合一级反应。在pH 3.6-12.0范围内，在2537 A下灭活的量子产率为0.024。失活伴随着光氧化和其他次级过程，没有明显的游离氨基酸或肽的释放。本文介绍了一种用Beckman分光光度计测定溶菌酶活力的快速、重复性好的方法。
The u.-v. inactivation of lysozyme conformed to a 1st order reaction. The quantum yield for inactivation at 2537 A was 0.024 at pH range of 3.6-12.0. The inactivation was accompanied by photooxidation and other secondary processes, with no apparent liberation of free amino acids or peptides. A rapid and reproducible method for measuring lysozyme activity, based on the degree of the digestion of Micrococcus lvsodeikticus cells in a Beckman spectrophotometer was descr.