Author Correction: Photocatalytic proximity labelling of MCL-1 by a BH3 ligand
Author Correction: Photocatalytic proximity labelling of MCL-1 by a BH3 ligand
复制标题
作者更正：BH3 配体对 MCL-1 进行光催化邻近标记
DOI：
10.1038/s42004-019-0243-z
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发表时间：
2019
影响因子：
5.9
通讯作者：
Beard H
中科院分区：
文献类型：
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作者：
Beard H
配体导向的蛋白质标记允许在蛋白质上引入不同的化学官能团，而不需要遗传编码的标签。在这里，我们报告了一种方法，用于快速标记的蛋白质，使用钌-联吡啶（Ru（II）（bpy）3）-修饰的肽，旨在模仿相互作用的BH 3配体内的BCL-2家族蛋白质-蛋白质相互作用。使用亚化学计量量的（Ru（II）（bpy）3）-修饰的NOXA-B和可见光照射1分钟，抗凋亡蛋白MCL-1可以与各种功能标签的光标记。与以前的报告Ru（II）（联吡啶）3-介导的光标记，串联质谱实验表明，标记位点是一个半胱氨酸残基的MCL-1。MCL-1可以选择性地与其他蛋白质混合标记，包括结构相关的BCL-2成员BCL-xL。这些结果表明，邻近诱导的光标记是适用于介导蛋白质-蛋白质相互作用的接口，并为未来使用配体导向的邻近标记BCL-2家族蛋白质的相互作用组的动态分析铺平了道路。
Ligand-directed protein labelling allows the introduction of diverse chemical functionalities onto proteins without the need for genetically encoded tags. Here we report a method for the rapid labelling of a protein using a ruthenium-bipyridyl (Ru(II)(bpy)3)-modified peptide designed to mimic an interacting BH3 ligand within a BCL-2 family protein-protein interactions. Using sub-stoichiometric quantities of (Ru(II)(bpy)3)-modified NOXA-B and irradiation with visible light for 1 min, the anti-apoptotic protein MCL-1 can be photolabelled with a variety of functional tags. In contrast with previous reports on Ru(II)(bpy)3-mediated photolabelling, tandem mass spectrometry experiments reveal that the labelling site is a cysteine residue of MCL-1. MCL-1 can be labelled selectively in mixtures with other proteins, including the structurally related BCL-2 member, BCL-xL. These results demonstrate that proximity-induced photolabelling is applicable to interfaces that mediate protein-protein interactions, and pave the way towards future use of ligand-directed proximity labelling for dynamic analysis of the interactome of BCL-2 family proteins.