Comparison of effects on macrophage cultures of glass fibre, glass powder, and chrysotile asbestos
Comparison of effects on macrophage cultures of glass fibre, glass powder, and chrysotile asbestos
复制标题
玻璃纤维、玻璃粉和温石棉对巨噬细胞培养物的影响比较
DOI：
10.1136/oem.29.3.280
复制
发表时间：
1972
期刊：
影响因子：
--
通讯作者：
N. Manojlovic
中科院分区：
文献类型：
--
作者：
E. Beck;P. Holt;N. Manojlovic
Beck，E. G.，霍尔特，P.F.，Manojlović，N.（1972）.Brit. J. industry. Med.，29，280-286.玻璃纤维、玻璃粉和温石棉对巨噬细胞培养的影响比较。比较了玻璃纤维、玻璃粉和温石棉对巨噬细胞培养的影响。玻璃纤维在增加培养的巨噬细胞的细胞膜通透性方面表现得像温石棉。这可以通过上清液中乳酸脱氢酶活性的增加来证明。通过乳酸盐的产生来测量的代谢并不像石英被吞噬时那样减少。玻璃粉的行为类似于惰性粉尘，对赤藓红B染色的细胞数量几乎没有影响，而乳酸脱氢酶活性略有增加，两者均在对照范围内。由于吞噬作用导致的较高代谢，乳酸盐产量增加。粉尘可产生两种基本效应，即毒性效应和细胞膜渗透性的变化。由于长纤维的缓慢且有时不完全的摄取过程，对细胞膜的非特异性影响可能是形态学的函数，特别是纤维的长度。某些粉尘与细胞膜接触后立即产生一种主要的特异性效应。
Beck, E. G., Holt, P. F., and Manojlović, N. (1972).Brit. J. industr. Med.,29, 280-286. Comparison of effects on macrophage cultures of glass fibre, glass powder, and chrysotile asbestos. The effects on macrophage cultures of glass fibre, glass powder, and chrysotile asbestos are compared. Glass fibre behaves like chrysotile in producing an increase in cell membrane permeability in cultured macrophages. This is demonstrable by the increase in lactic dehydrogenase activity in the supernatant fluid. The metabolism, measured by lactate production, is not reduced as it is when quartz is phagocytosed. Glass powder behaves like the inert dust corundum, producing little change in the number of cells stained by erythrosin B and a small increase in lactate dehydrogenase activity, both being in the range of the control. There is an increase in lactate production as a result of higher metabolism due to phagocytosis. Dusts may produce two basic effects, namely a toxic effect and change in cell membrane permeability. A non-specific effect on the cell membrane due to the slow and sometimes incomplete process of ingestion of long fibres is probably a function of the morphology, particularly the length of the fibres. A primary specific effect induced by some dusts immediately follows contact with the cell membrane.