Matrix metalloproteinase-13 expression in rabbit knee joint connective tissues: influence of maturation and response to injury
Matrix metalloproteinase-13 expression in rabbit knee joint connective tissues: influence of maturation and response to injury
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DOI：
10.1016/s0945-053x(00)00093-7
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发表时间：
2000-09-01
期刊：
影响因子：
6.9
通讯作者：
Hart, DA
中科院分区：
文献类型：
--
作者：
Le Graverand, MPH;Eggerer, J;Hart, DA
本研究假设基质金属蛋白酶-13(MMP13，胶原酶-3)在韧带成熟、瘢痕愈合和关节不稳定等重要的基质重塑过程中被诱导表达。因此，我们评估了在组织成熟和愈合的不同情况下，内侧副韧带(MCL)中的基质金属蛋白酶-13的表达。分别于损伤后3周和8周检测前交叉韧带切断后关节内3种结缔组织(关节软骨、半月板和滑膜)中基质金属蛋白酶-13的表达。免疫组织化学和Western blotting检测到基质金属蛋白酶13mRNA(半定量RT-PCR)和蛋白表达(免疫组织化学和Western blotting)。组织成熟早期(子宫内29天和2月龄)较后期(5月龄和12月龄)检测到较高的MCH、MMP13基因表达水平。这种表达模式在MCL损伤后重现，在损伤后3周在瘢痕组织中表达非常高，然后在14周时下降到与对照组没有显著差异的水平。在前交叉韧带横断后和内侧副韧带愈合过程中，半月板和滑膜中MMP-13蛋白水平的升高与mRNA水平的升高有关。这些结果表明，基质金属蛋白酶-13的表达受许多变量的调节，在结缔组织重塑非常活跃的情况下会出现高水平的表达。(C)2000年Elsevier Science B.V./国际基质生物学学会。版权所有。
The hypothesis of the present work was that expression of matrix metalloproteinase-13 (MMP-13, collagenase-3) would be induced during conditions involving important matrix remodeling such as ligament maturation, scar healing and joint instability. Therefore, MMP-13 expression in the medial collateral ligament (MCL) during the variable situations of tissue maturation and healing was assessed. MMP-13 expression in three intra-articular connective tissues of the knee (i.e. articular cartilage, menisci and synovium) following the transection of the anterior cruciate ligament of the knee was evaluated at 3 and 8 weeks post-injury. MMP-13 mRNA (semi-quantitative RT-PCR) and protein (immunohistochemistry and Western blotting) were detected in all of the tissues studied. Significantly higher :MCH, mRNA levels for MMP-13 were detected during the early phases of tissue maturation (i.e. 29 days in utero and 2-month-old rabbits) compared to later phases (5- and 12-month-old rabbits). This pattern of expression was recapitulated following MCL injury, with very high levels of expression in scar tissue at 3 weeks post-injury and then a decline to levels not significantly different from control values by 14 weeks. Elevated mRNA levels correlated with increased protein levels for MMP-13 in both menisci and synovium following the transection of the anterior cruciate ligament and during medial collateral ligament healing. These results indicate that MMP-13 expression is regulated by a number of variables and that high levels of expression occur in situations when connective tissue remodeling is very active. (C) 2000 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.