CONTROL OF JUNCTIONAL ACETYLCHOLINESTERASE BY NEURAL AND MUSCULAR INFLUENCES IN THE RAT
CONTROL OF JUNCTIONAL ACETYLCHOLINESTERASE BY NEURAL AND MUSCULAR INFLUENCES IN THE RAT
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DOI：
10.1113/jphysiol.1980.sp013280
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发表时间：
1980-01-01
影响因子：
5.5
通讯作者：
SLATER, CR
中科院分区：
文献类型：
--
作者：
LOMO, T;SLATER, CR
在成年大鼠中研究了移植的外来神经（腓浅神经）和去神经支配的比目鱼肌之间形成的异位神经肌肉接头处 AChE [乙酰胆碱酯酶] 的发育。比目鱼神经切断后 6-7 天以及传播开始后 3-4 天，腓骨神经芽附近开始出现交界 AChE 活性。比目鱼肌神经被切断后0-4天，当腓神经被切断时，没有出现组织化学可检测到的AChE。根据抑制剂研究确定，直接电刺激去神经支配的比目鱼肌会导致真正的 AChE 斑块出现在比目鱼肌神经被切断 2-4 天后腓骨神经被切断的肌肉中，但不会出现在同时切断 2 条神经的肌肉中。这些斑块仅出现在腓神经芽附近，并且与新形成但稳定的乙酰胆碱敏感性峰值一致。局部应用新斯的明可延长并增强这些部位由乙酰胆碱脉冲引起的去极化反应。在腓神经完整的肌肉中，AChE 斑块在几周内逐渐从不成熟的外观转变为正常终板特征的成熟外观。在腓神经被切断的受刺激肌肉中，斑块染色强烈，但形态上仍不成熟。肌肉活动可能对于神经肌肉接头发育中乙酰胆碱酯酶的出现很重要。乙酰胆碱酯酶可能仅积聚在肌肉表面神经纤维与肌肉纤维接触时留下痕迹的部位。这些痕迹很快形成，并在神经-肌肉相互作用后短短两天内持续存在。肌肉似乎是连接性乙酰胆碱酯酶的主要来源，因为刺激肌肉会在神经退化的肌肉中诱导强烈的乙酰胆碱酯酶活性。
The development of AChE [acetylcholinesterase] at ectopic neuromuscular junctions forming between a transplanted foreign nerve (the superficial fibular nerve) and the denervated soleus muscle was studied in adult rats. Junctional AChE activity began to appear in the vicinity of the fibular nerve sprouts 6-7 days after section of the soleus nerve and 3-4 days after the onset of transmission. No histochemically detectable AChE appeared when the fibular nerve was cut 0-4 days after the soleus nerve had been cut. Direct electrical stimulation of the denervated soleus muscle caused plaques of true AChE, as determined by inhibitor studies, to appear in muscles where the fibular nerve had been cut 2-4 days after the soleus nerve but not in muscles where the 2 nerves were cut at the same time. The plaques appeared only in the vicinity of fibular nerve sprouts and coincided with newly formed but stable peaks of ACh sensitivity. Local application of Neostigmine prolonged and increased the depolarizing responses evoked by pulses of ACh at these sites. In muscles where the fibular nerve was intact the AChE plaques changed gradually over a few weeks from an immature appearance to a mature appearance characteristic of normal end-plates. In stimulated muscles where the fibular nerve was cut the plaques stained intensely but remained morphologically immature. Muscle activity is probably important for the appearance of AChE at developing neuromuscular junctions. AChE may accumulate only at sites on the muscle surface where the nerve fibres have left a trace upon contact with the muscle fibers. These traces form quickly and persist after nerve-muscle interaction of as little as 2 days. The muscle appears as a major source of junctional AChE since stimulation of the muscle induces intense AChE activity in muscles where the nerve has degenerated.