EFFICACY OF A RIFT-VALLEY FEVER VIRUS-VACCINE AGAINST AN AEROSOL INFECTION IN RATS
EFFICACY OF A RIFT-VALLEY FEVER VIRUS-VACCINE AGAINST AN AEROSOL INFECTION IN RATS
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DOI：
10.1016/0264-410x(91)90285-e
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发表时间：
1991-10-01
期刊：
影响因子：
5.5
通讯作者：
MEADORS, G
中科院分区：
文献类型：
--
作者：
ANDERSON, GW;LEE, JO;MEADORS, G
将福尔马林灭活的裂谷热病毒（RVFV）疫苗TSI-GSD-200皮下给予高度易感的成年Wistar-Furth大鼠（LD 50 -1 p.f.u.，ZH 501菌株）。 在第0、7和28天接种疫苗，与高危人员使用的时间相同。 免疫后6个月，当血清空斑减少中和滴度（PRNT）80降至较低或无法检测的水平时，用4.4 log 10 p.f.u. ZH 501强毒株的鼻用动态气溶胶装置。 97%（33/34）的未接种对照大鼠死亡。 相比之下，只有32%（33/105）的接种动物死亡。 在接种疫苗的死亡大鼠中，死亡的平均时间增加了一倍，死亡原因从肝炎转变为脑炎。 PRNT 80大于或等于1：40的大鼠免于临床疾病和肝脏或脑炎病变的组织学证据。 虽然针对气溶胶攻击的免疫力的精确机制尚未解决，但血清PRNT滴度与保护相关。
The formalin-inactivated Rift Valley fever virus (RVFV) vaccine, TSI-GSD-200, was administered subcutaneously to highly susceptible adult Wistar-Furth rats (LD50-1 p.f.u., ZH501 strain). Vaccine was administered on days 0, 7 and 28, the same time course used for at-risk personnel. Six months postimmunization, when the serum plaque-reduction neutralization titre (PRNT)80 had declined to low or undetectable levels, rats were challenged with 4.4 log10 p.f.u. of the virulent ZH501 strain in a nose-only dynamic aerosol apparatus. Ninety-seven per cent (33/34) of the non-vaccinated control rats died. In contrast, only 32% (33/105) of the vaccinated animals died. In vaccinated rats that succumbed, there was a doubling of the mean time to death and the cause of death shifted from hepatitis to encephalitis. Rats with a PRNT80 of greater-than-or-equal-to 1:40 were protected from clinical disease and histological evidence of hepatic or encephalitic lesions. While the precise mechanisms of immunity against aerosol challenge remain unresolved, here the serum PRNT titre correlated with protection.