Rabbit hepatitis E virus is an opportunistic pathogen in specific-pathogen-free rabbits with the capability of cross-species transmission
Rabbit hepatitis E virus is an opportunistic pathogen in specific-pathogen-free rabbits with the capability of cross-species transmission
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兔戊型肝炎病毒是无特异性病原体家兔的机会致病菌，具有跨种传播能力
DOI：
10.1016/j.vetmic.2016.10.029
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发表时间：
2017-03-01
影响因子：
3.3
通讯作者：
Zhao, Qin
中科院分区：
文献类型：
--
作者：
Liu, Baoyuan;Sun, Yani;Zhao, Qin
戊型肝炎病毒（HEV）已被发现在兔子，最近确定的天然水库。在这项研究中，抗戊型肝炎病毒的抗体和病毒RNA检测来自中国陕西省的一个无特定病原体（SPF）兔供应商的兔子。本文克隆的部分戊型肝炎病毒ORF 2基因的BLAST结果表明，两种病毒株在兔子中传播。对其中一株的全基因组（7302 bp）和另一株的部分ORF 1基因（1537 bp）进行了序列测定，结果表明，它们的同源性为90%，与其它已知的兔戊型肝炎病毒的同源性为78%~ 94%。此外，接种兔戊型肝炎病毒从SPF兔在这里研究导致感染SPF猪，这种跨种属传播证明了血清转化，病毒血症和粪便病毒脱落。这些结果表明，为了防止这种人畜共患病原体的传播，应在SPF供应商设施中对家兔进行常规HEV RNA检测。(C)2017爱思唯尔B. V.保留所有权利。
Hepatitis E virus (HEV) has been detected in rabbits, a recently identified natural reservoir. In this study, anti-HEV antibodies and viral RNA were detected in rabbits sourced from a specific-pathogen-free (SPF) rabbit vendor in Shaanxi Province, China. BLAST results of partial HEV ORF2 genes cloned here indicated that two viral strains circulated in the rabbits. Sequence determination of the complete genome (7302 bp) of one strain and a partial ORF1 gene (1537 bp) of the other strain showed that they shared 90% identity with one another and 78%-94% identity with other known rabbit HEVs. In addition, inoculation with rabbit HEV from SPF rabbits studied here resulted in infection of SPF pigs; this cross-species transmission was evidenced by seroconversion, viremia and faecal virus shedding. These results suggest that to prevent spread of this zoonotic pathogen, rabbits should be tested routinely for HEV RNA in SPF vendor facilities. (C) 2017 Elsevier B.V. All rights reserved.