(Na,K)-ATPase and Ouabain binding in reticulocytes from dogs with high K and low K erythrocytes and their changes during maturation.
(Na,K)-ATPase and Ouabain binding in reticulocytes from dogs with high K and low K erythrocytes and their changes during maturation.
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(Na,K)-ATP酶和哇巴因在高K和低K红细胞狗的网织红细胞中的结合及其在成熟过程中的变化。
DOI：
10.1016/s0021-9258(19)83625-6
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
M. Inaba
中科院分区：
文献类型：
--
作者：
Y. Maede;M. Inaba
本研究表明，狗网织红细胞有相当数量的（Na，K）-ATP酶，但在成熟为红细胞的过程中迅速丢失。此外，来自具有以高（Na，K）-ATP酶活性和高K、低Na浓度为特征的红细胞的狗的网织红细胞（HK狗; Maede，Y.，Inaba，M.，和Taniguchi，N.（1983）Blood 61，493 -499）比来自正常狗（LK狗）的细胞具有更多的哇巴因结合位点。我们的结果如下：i）在LK和HK犬红细胞中，哇巴因结合平衡时的最大结合容量（Bmax）分别约为0和1，500个结合位点/细胞。ii）LK犬的网织红细胞具有约5，700个哇巴因结合位点/细胞。iii）HK网织红细胞中哇巴因的Bmax值为约10，000位点/细胞，是LK网织红细胞中的2倍。iv）每种类型的网织红细胞中的24 Na和42 K的哇巴因敏感通量与细胞上的哇巴因结合位点的数量相容。v）LK狗的网织红细胞中的哇巴因结合能力以及（Na，K）-ATP酶活性在成熟为红细胞期间迅速下降至几乎为零。（vi）虽然HK犬网织红细胞在成熟过程中也表现出类似的（Na，K）-ATP酶的退化，但它们在成熟后仍保留一定数量的哇巴因结合位点，导致HK红细胞膜上（Na，K）-ATP酶的高活性。
The present study demonstrated that dog reticulocytes had considerable amounts of (Na,K)-ATPase, but lost it rapidly during maturation into erythrocytes. Furthermore, reticulocytes from dogs possessing erythrocytes characterized with high (Na,K)-ATPase activity and high K, low Na concentrations (HK dogs; Maede, Y., Inaba, M., and Taniguchi, N. (1983) Blood 61,493-499) had more ouabain binding sites than cells from normal dogs (LK dogs). Our results were as follows: i) The maximal binding capacities (Bmax) for ouabain binding at equilibrium were approximately 0 and 1,500 binding sites/cell in LK and HK dog erythrocytes, respectively. ii) Reticulocytes from LK dogs possess approximately 5,700 ouabain binding sites/cell. iii) The Bmax value for ouabain in HK reticulocytes was about 10,000 sites/cell, being 2-fold that in LK reticulocytes. iv) Ouabain-sensitive fluxes of 24Na and 42K in each type of reticulocyte were compatible with the number of ouabain binding sites on the cells. v) Ouabain binding capacity, as well as (Na,K)-ATPase activity, in the reticulocytes from LK dogs fell rapidly to nearly zero during the maturation into erythrocytes. vi) Although reticulocytes from HK dogs also showed a similar regression of (Na,K)-ATPase during maturation, they retained a certain number of ouabain binding sites even after maturation, resulting in the high activity of (Na,K)-ATPase in HK erythrocyte membrane.
大鼠网织红细胞中的钾通量。
DOI：
10.1016/0005-2736(81)90167-x
发表时间：
1981
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Furukawa,H;Bilezikian,JP;Loeb,JN
通讯作者：
Loeb,JN
在朋友红白血病细胞中，质膜结合蛋白激酶对 (Na,K)-ATP 酶进行磷酸化。
DOI：
--
发表时间：
1983
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yeh,LA;Ling,L;English,L;Cantley,L
通讯作者：
Cantley,L