RNA interference: applicability in tick research
RNA interference: applicability in tick research
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DOI：
10.1023/a:1025346131903
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发表时间：
2002-01-01
影响因子：
2.2
通讯作者：
Essenberg, RC
中科院分区：
文献类型：
--
作者：
Aljamali, MN;Sauer, JR;Essenberg, RC
新的遗传工具的快速发展推动了基因发现机制的发展。RNA干扰（RNAi）是一种基因沉默技术，近年来被广泛应用于许多真核生物中，以阐明未知基因的功能和生物化学途径。我们在美洲无眼蜱雌性蜱中使用dsRNA技术来测试RNAi方法在蜱中的适用性。在体外孵育蜱唾液腺（TSG）和在体内将组胺结合蛋白（HBP）dsRNA注射到整个雌性蜱中导致dsRNA处理组中HBP转录物的减少。与对照蜱相比，注射dsRNA的蜱的摄食模式存在显著差异，这可能反映了摄食部位局部组胺浓度的增加。据我们所知，这是第一个在蜱虫中进行的RNAi研究。总之，RNAi可以应用于蜱，并可能用于测试关键蛋白的功能，这些蛋白对于在蜱-宿主界面上避免宿主防御至关重要。
The rapid development of new genetic tools has boosted the gene discovery machinery. RNA interference (RNAi), a gene silencing process, has been recently used in several eukaryotic organisms to elucidate the function(s) of unknown genes and biochemical pathways. We used the dsRNA technique in Amlyomma americanum female ticks to test the applicability of the RNAi approach in ticks. Incubation of tick salivary glands (TSGs) in vitro and in vivo injection into whole female ticks with histamine binding protein (HBP) dsRNA led to a reduction in the HBP transcripts in the dsRNA treated groups. The dsRNA-injected ticks had a profound difference in their feeding pattern compared to control ticks that might reflect an increase in local histamine concentrations at the feeding sites. To our knowledge, this is the first RNAi study in ticks. In conclusion, RNAi can be applied in ticks and might be used to test the function of key proteins crucial for avoiding host defense at the tick-host interface.