Transcriptomic Analysis Reveals Differentially Expressed Genes Associated with Pine Wood Nematode Resistance in Resistant Pinus thunbergii.
Transcriptomic Analysis Reveals Differentially Expressed Genes Associated with Pine Wood Nematode Resistance in Resistant Pinus thunbergii.
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转录组分析揭示了抗性黑松中与松材线虫抗性相关的差异表达基因。
DOI：
10.1093/treephys/tpad018
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发表时间：
2023
期刊：
影响因子：
4
通讯作者：
Yan Zhang
中科院分区：
文献类型：
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作者：
Xinyan Wang;Xiaoqin Wu;Tong Wen;Yaqi Feng;Yan Zhang
松树枯萎病（PWD）（由线虫引起）对东亚松林危害极大。黑松是一种低抗性的松树，对松材线虫病的易感性高于赤松和马尾松。通过田间接种抗、感白绢病菌株，分析了接种后24 h转录谱的差异。我们鉴定了2，603个差异表达基因（DEG），在PWN敏感的P. thunbercavity，而2，559个DEG在PWN抗性的P. thunbercavity。接种前，抗、感松材线虫菌株的DEG主要集中在氧化还原酶活性途径（106 DEG），其次是氧化还原酶活性途径（152 DEG）;接种松材线虫后，DEG主要集中在氧化还原酶活性途径（119 DEG），其次是氧化还原酶活性途径（84 DEG）。接种前，代谢途径分析结果发现，苯丙素代谢途径基因表达上调，木质素合成途径基因表达丰富;木质素合成相关的肉桂酰辅酶A还原酶（CCR）编码基因表达上调，而木质素合成相关基因表达下调，且抗性植株木质素含量始终高于感病植株。这些结果揭示了抗、感鼠伤寒沙门氏菌在应对松材线虫感染时的不同策略。
Pine wilt disease (PWD) (caused by the nematode Bursaphelenchus xylophilus) is extremely harmful to pine forests in East Asia. As a low-resistance pine species, Pinus thunbergii is more vulnerable to pine wood nematode (PWN) than Pinus densiflora and Pinus massoniana. Field inoculation experiments were conducted on PWN-resistant and susceptible P. thunbergii, and the difference in transcription profiles 24 hours after inoculation was analyzed. We identified 2,603 differentially expressed genes (DEGs) in PWN-susceptible P. thunbergii, while 2,559 DEGs were identified in PWN-resistant P. thunbergii. Before inoculation, DEGs between PWN-resistant and PWN-susceptible P. thunbergii were enriched in the REDOX activity pathway (152 DEGs), followed by the oxidoreductase activity pathway (106 DEGs); After inoculation with PWN, however, the opposite was observed, DEGs were enriched in the oxidoreductase activity pathway (119 DEGs), followed by the REDOX activity pathway (84 DEGs). Before inoculation, according to the metabolic pathway analysis results, we found more genes upregulated in phenylpropanoid metabolic pathways and enriched in lignin synthesis pathways; cinnamoyl-CoA reductase (CCR)-coding genes related to lignin synthesis were upregulated in PWN-resistant P. thunbergii and downregulated in PWN-susceptible P. thunbergii, and the lignin content was always higher in resistant than in susceptible P. thunbergii. These results reveal distinctive strategies of resistant and susceptible P. thunbergii in dealing with PWN infections.