Altered Expression of Connective Tissue Genes in Postnatal Chondroinduced Human Dermal Fibroblasts
Altered Expression of Connective Tissue Genes in Postnatal Chondroinduced Human Dermal Fibroblasts
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DOI：
10.1080/03008200390223891
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发表时间：
2003-01
影响因子：
2.9
通讯作者：
K. Yates;J. Glowacki
中科院分区：
文献类型：
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作者：
K. Yates;J. Glowacki
在一个新的模型，出生后软骨诱导，正常人皮肤成纤维细胞（hDF）培养与脱矿骨粉（DBP）表达软骨细胞的特征后7天。代表性差异分析（RDA）之前，明显的成软骨细胞（3天）显示结缔组织基因（胶原蛋白，胶原蛋白受体，和翻译后酶）的表达改变。北方或RT-PCR分析在3，7，14，或21天显示不同的表达模式的基因。COL 3A 1瞬时上调，而COL 11 A1、整合素f-11、赖氨酰氧化酶和赖氨酰羟化酶2 mRNA的上调持续7天。核心蛋白聚糖的下调持续21 d。与人皮肤和人关节软骨细胞相比，DBP诱导的翻译后酶的表达是独特的。因此，这些数据表明，一个“诱导成软骨细胞”的阶段，具有独特的结缔组织基因表达谱，可能会导致一个基质支持软骨形成的出生后细胞。
In a novel model for postnatal chondroinduction, normal human dermal fibroblasts (hDFs) cultured with demineralized bone powder (DBP) express chondrocyte features after 7 days. Representational difference analysis (RDA) prior to overt chondroblastogenesis (3 days) revealed altered expression of connective tissue genes (collagens, collagen receptors, and post-translational enzymes). Northern or RT-PCR analysis at 3, 7, 14, or 21 d showed different expression patterns for those genes. COL3A1 was transiently upregulated, whereas upregulation of COL11A1, integrin f -11, lysyl oxidase, and lysyl hydroxylase 2 mRNAs persisted for 7 days. Downregulation of decorin was sustained for 21 d. The expression of the post-translational enzymes induced by DBP was unique when compared with human skin and human articular chondrocytes. Thus, the data suggest an "induced chondroblast" stage with a unique connective tissue gene expression profile that may result in a matrix supportive of chondrogenesis by postnatal cells.