Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae)
Storage behavior and changes in concentrations of abscisic acid and gibberellins during dormancy break and germination in seeds of Phellodendron amurense var. wilsonii (Rutaceae)
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DOI：
10.1093/treephys/tpp111
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发表时间：
2010-02-01
期刊：
影响因子：
4
通讯作者：
Baskin, Carol C.
中科院分区：
文献类型：
--
作者：
Chen, Shun-Ying;Chien, Ching-Te;Baskin, Carol C.
众所周知，亚洲药用植物黄柏含有几种非常重要的具有生物作用的化合物。本研究的主要目的是确定黄柏种子是否存在变异。威森尼可以储存并表征它们的休眠状态。该分类群的种子在-20℃和-80℃以及液氮中贮藏时保持了很高的发芽率，表明它们具有正统的储存行为。从新鲜果实中采集的完整种子处于休眠状态，需要在4摄氏度下进行12周的冷层积才能完全萌发。剥离种皮对打破种子休眠有一定效果。外源赤霉素(GA(3)、GA(4)和GA(4+7))促进种子萌发，其中GA(4)和GA(4+7)比GA(3)更有效。脱落酸(ABA)生物合成抑制剂氟里酮在打破休眠方面是有效的，但其效果不如单独使用GA(4)或GA(4+7)。GA生物合成抑制剂多效唑对种子萌发有抑制作用，GA(4)和GA(4+7)可完全逆转这种抑制作用。与新鲜种子相比，冷层积或35/10℃孵化促进种子萌发的ABA含量下降了约4-6倍。GA(3)、GA(4)和GA(7)在非休眠种子和胚根萌发的种子中的含量均高于新鲜种子。目前的结果似乎表明，山杨的休眠。威森尼种子部分由种皮形成，部分由高ABA含量形成。萌发过程中ABA含量下降，GA(3)、GA(4)和GA(7)含量上升。
The medicinal Asian plant genus Phellodendron is known to contain several very important compounds that have biological action. The main purpose of this study was to determine whether seeds of Phellodendron amurense var. wilsonii can be stored and to characterize their dormancy. Seeds of this taxon stored at -20 and -80 degrees C and in liquid nitrogen retained their high germinability, indicating that they have orthodox storage behavior. Intact seeds from freshly collected fruits were dormant and required 12 weeks of cold stratification at 4 degrees C for complete germination. Scarifying the seed coat was partially effective in breaking seed dormancy. Exogenous gibberellins (GA(3), GA(4) and GA(4+7)) promoted germination of scarified seeds, GA(4) and GA(4+7) being more effective than GA(3). Fluridone, an abscisic acid (ABA) biosynthesis inhibitor, was efficient in breaking dormancy, but it was less effective than GA(4) or GA(4+7) alone. Paclobutrazol, a GA biosynthesis inhibitor, inhibited seed germination, and the inhibitory effect was reversed completely by GA(4) and by GA(4+7). ABA content of seeds Subjected to cold stratification or to incubation at 35/10 degrees C, which enhanced seed germination, was reduced about four- to sixfold compared to that of fresh seeds. Higher concentrations of GA(3), GA(4) and GA(7) were detected in nondormant seeds and in seeds with an emerged radicle than in fresh seeds. Present results seem to indicate that dormancy in P. amurense var. wilsonii seeds is imposed partially by the seed coat and partially by high ABA content. ABA content decreased and GA(3), GA(4) and GA(7) content increased during germination.