Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.
Cells nonproductively transformed by Abelson murine leukemia virus express a high molecular weight polyprotein containing structural and nonstructural components.
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由阿贝尔森鼠白血病病毒非生产性转化的细胞表达含有结构和非结构成分的高分子量多蛋白。
DOI：
10.1073/pnas.75.8.3974
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发表时间：
1978
影响因子：
11.1
通讯作者：
J. Stephenson
中科院分区：
文献类型：
--
作者：
F. Reynolds;T. L. Sacks;D. Deobagkar;J. Stephenson
通过竞争免疫测定法分析由复制缺陷型小鼠白血病病毒 (AbLV) 的 Abelson 株非生产性转化的细胞克隆的 C 型病毒抗原表达。 AbLV 转化的水貂非生产品系被发现表达 110,000 至 130,000 分子量的多蛋白，其中含有与分子量约为 80,000-100,000 的非结构 AbLV 编码成分共价连接的鼠白血病病毒 gag 蛋白 p15 和 p12。这种多蛋白与其他病毒体编码的 gag 基因蛋白（例如 p30、p10、病毒逆转录酶（RNA 依赖性 DNA 聚合酶）或主要病毒包膜糖蛋白 gp70）缺乏可检测到的抗原交叉反应性。通过与猫和禽肉瘤病毒的早期数据类比，这些结果表明，这种多蛋白的一部分可能代表 AbLV src 基因产物，并且在翻译中它最初以前体形式与 gag 结构蛋白连接。用野生小鼠兼嗜性 C 型病毒分离株 4070-A 或内源性猫病毒 RD114 对经 AbLV 非生产性转化的水貂细胞进行重复感染，导致产生含有高浓度 AbLV 编码的前体多蛋白的假型病毒。
Cell clones nonproductively transformed by the replication-defective Abelson strain of murine leukemia virus (AbLV) were analyzed for type C viral antigen expression by competition immunoassay. AbLV-transformed mink non-producer lines were found to express a 110,000- to 130,000-molecular weight polyprotein containing murine leukemia virus gag proteins p15 and p12 covalently linked to nonstructural AbLV-coded component(s) of around 80,000-100,000 molecular weight. This polyprotein lacked detectable antigenic cross-reactivity with other virion-coded gag gene proteins such as p30, p10, the viral reverse transcriptase (RNA-dependent DNA polymerase), or the major viral envelope glycoprotein, gp70. By analogy to earlier data on feline and avian sarcoma viruses, these results suggest that a portion of this polyprotein might represent the AbLV src gene product and that in translation it is initially linked in precursor form to gag structural proteins. Superinfection of mink cells nonproductively transformed by AbLV--with either a wild mouse amphotropic type C virus isolate, 4070-A, or with the endogenous cat virus, RD114--led to production of pseudotype virus containing high concentrations of the AbLV-coded precursor polyprotein.