Mild-intensity running exercise recovered motor function by improvement of ankle mobility after unilateral brain injury of mice using three-dimensional kinematic analysis techniques
Mild-intensity running exercise recovered motor function by improvement of ankle mobility after unilateral brain injury of mice using three-dimensional kinematic analysis techniques
复制标题
DOI：
10.1016/j.brainres.2022.148160
复制
发表时间：
2022-11-17
期刊：
影响因子：
2.9
通讯作者：
Izumizaki，Masahiko
中科院分区：
文献类型：
--
作者：
Yoshikawa，Akira;Ohtaki，Hirokazu;Izumizaki，Masahiko
运动功能障碍，如步态障碍，是由创伤性脑损伤或中风引起的一种主要残疾。在临床和实验中，跑步机跑经常被用作一种身体锻炼(Ex)，用于患者的康复。在动物实验中，虽然动态行为缺陷可以使用评分系统进行评估，但局部和次要行为很难确定。本研究旨在利用三维(3D)运动学分析来评估小鼠在有或没有低强度跑步Ex的情况下的运动功能障碍和脑损伤(BD)后的恢复。为确定运动强度，对C57/BL6品系雄性幼年小鼠进行递增跑试验，同时测定O2和CO2的肺气体交换。然后将动物作为BD进行左侧皮质切除，部分小鼠进行Ex(10分钟/分钟，30分钟/周，5次/周)，持续4周。用4台同步摄像机记录有Ex和BD的BD组和无(w/o)Ex的假手术组(Sham)小鼠的步态，并通过三维运动学分析评估步态。BD W/O Ex小鼠与假W/O Ex小鼠相比，双腿的步幅、步幅和步幅宽度有显著差异。与Ex小鼠相比，BD有明显改善。通过轨迹分析，BD w/o Ex小鼠的踝关节运动受限，背部/平台屈曲受损。与这些损伤相一致的是，健侧也表现出不同的轨迹，提示代偿性运动。提示BD患者运动功能恢复后应给予适当的Ex。此外，本研究表明，3D运动学分析是检测由于患侧受损和健侧补偿而引起的轻微行为改变的有力工具。
Motor dysfunction, such as gait impairment, is a major disability induced by traumatic brain injury or stroke. Treadmill running is often used as a physical exercise (Ex) clinically and experimentally for the recovery of patients. In animal experiments, although dynamic behavioral deficits can be evaluated using scoring systems, local and minor behaviors are difficult to determine. This study aims to evaluate motor dysfunction and recovery after brain damage (BD) with/without mild-intensity running Ex in mice using three-dimensional (3D) kinematic analysis. To determine exercise intensity, C57/BL6-strain male young adult mice were examined in an incremental running test while the pulmonary gas exchange of O2and CO2were measured. The animals were then subjected to left hemidecortication as BD, and some mice performed Ex (10 m/min for 30 min 5 times/wk) for 4 weeks. The BD with Ex and BD or sham-operated mice (sham) without (w/o) Ex had their gait recorded by four synchronized cameras, and gait was evaluated via 3D-kinematic analysis. The BD w/o Ex mice significantly differed in stride, step, and stride width for both limbs compared to the sham w/o Ex mice. The BD with Ex mice showed improvement. The BD w/o Ex mice had restricted ankle movements and impairment in dorsal/planter flexing using trajectory analysis. Consistent with these impairments, the nonaffected side also exhibited a different trajectory, suggesting compensatory movements. These results suggest that the appropriate Ex after BD recovered motor function. Furthermore, the present study suggested that 3D-kinematic analysis is a powerful tool for detecting minor behavioral alterations owing to the impairment of the affected side and the compensation of the unaffected side.