A PROTEIN PHOSPHATASE 2C INVOLVED IN ABA SIGNAL-TRANSDUCTION IN ARABIDOPSIS-THALIANA
A PROTEIN PHOSPHATASE 2C INVOLVED IN ABA SIGNAL-TRANSDUCTION IN ARABIDOPSIS-THALIANA
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DOI：
10.1126/science.8197457
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发表时间：
1994-06-03
期刊：
影响因子：
56.9
通讯作者：
GRILL, E
中科院分区：
文献类型：
--
作者：
MEYER, K;LEUBE, MP;GRILL, E
植物激素脱落酸（阿坝）介导多种反应，如气孔关闭，种子休眠的维持和植物生长的抑制。所有这三种反应都受到影响的ABA不敏感突变体abi1的拟南芥，这表明阿坝的信号传导的早期步骤是由ABI1基因座控制。通过染色体步移法克隆了ABI1基因，并在突变体的结构基因中发现了一个错义突变。ABI1基因编码的蛋白质与2C型丝氨酸或苏氨酸磷酸酶具有高度相似性，具有推定的Ca 2+结合位点的新特征。因此，ABI1产物对细胞信号传导组分的磷酸化状态的控制可以介导多效性激素反应。
The plant hormone abscisic acid (ABA) mediates various responses such as stomatal closure, the maintenance of seed dormancy, and the inhibition of plant growth. All three responses are affected in the ABA-insensitive mutant abi1 of Arabidopsis thaliana, suggesting that an early step in the signaling of ABA is controlled by the ABI1 locus. The ABI1 gene was cloned by chromosome walking, and a missense mutation was identified in the structural gene of the abi1 mutant. The ABI1 gene encodes a protein with high similarity to protein serine or threonine phosphatases of type 2C with the novel feature of a putative Ca2+ binding site. Thus, the control of the phosphorylation state of cell signaling components by the ABI1 product could mediate pleiotropic hormone responses.