Application of DNA amplification fingerprinting (DAF) to mixed culture bioreactors.
Application of DNA amplification fingerprinting (DAF) to mixed culture bioreactors.
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DNA 扩增指纹识别 (DAF) 在混合培养生物反应器中的应用。
DOI：
10.1007/bf01570059
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Sferra,PR
中科院分区：
文献类型：
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作者：
Breen,A;Rope,AF;Taylor,D;Loper,JC;Sferra,PR
对DNA扩增指纹图谱(DAF)作为生物反应器中混合微生物种群监测工具的应用进行了评价。在聚合酶链式反应(PCR)扩增过程中，使用短(8聚体或10聚体)寡核苷酸来启动从各种生物反应器中提取的DNA。本研究考察的反应器包括两套厌氧搅拌池连续流生物反应器。一套厌氧反应器在产甲烷条件下运行，另一套在硫酸盐还原条件下运行。甲醇进料反应器中的厌氧反应器群落显示出广泛的DAF同源性。还将DAF应用于固定膜偶氮染料降解反应器，以检测反应器中代表性区域基质的定植均匀度。该方法是一种在生物过程中监测微生物群落结构的快速和相对廉价的手段。由于不涉及样本的培育，群落的遗传图谱不会因生长条件而产生偏差。DAF概况可能有助于比较种群随时间的变化，或比较实验室规模的反应堆与试点规模的反应堆，但不足以评估群落多样性。
The use of DNA amplification fingerprinting (DAF) as a tool for monitoring mixed microbial populations in bioreactors was evaluated. Short (8-mer or 10-mer) oligonucleotides were used to prime DNA extracts from various biological reactors during polymerase chain reaction (PCR) amplification. The reactors examined in this study included two sets of anaerobic stirred tank continuous flow bioreactors. One set of anaerobic reactors was operated under methanogenic conditions and one set was operated under sulfate-reducing conditions. The anaerobic reactor communities in the methanol-fed reactors showed extensive DAF homology. DAF was also applied to a fixed-film azo dye degrading reactor to examine the degree of uniformity of colonization of the substratum in representative regions of the reactor. This method is a quick and relatively inexpensive means of monitoring microbial community structure during biological processes. Since no cultivation of the sample is involved, the genetic profile of the community is not biased by outgrowth conditions. DAF profiles may be useful for comparisons of population changes over time or of bench-scale vs pilot-scale reactors but not adequate for assessing community diversity.