Characterisation of the role and regulation of Ultrabithorax in sculpting fine-scale leg morphology.
Characterisation of the role and regulation of Ultrabithorax in sculpting fine-scale leg morphology.
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DOI：
10.3389/fcell.2023.1119221
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发表时间：
2023
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
Hox 基因在胚胎发生过程中表达，并决定动物体沿前后轴的区域特征。然而，它们还在胚胎后发挥作用，塑造精细的形态。为了更好地了解 Hox 基因如何整合到胚胎后基因调控网络中，我们进一步分析了 Ultrabithorax (Ubx) 在果蝇腿部发育过程中的作用和调控。 Ubx 调节第二对 (T2) 和第三对 (T3) 腿股骨上的鬃毛和毛状体图案的几个方面。我们发现，Ubx 对 T2 股骨近后区域毛状体的抑制可能是通过该 Hox 蛋白激活 microRNA-92a 和 microRNA-92b 的表达来介导的。此外，我们还发现了一种新的 Ubx 增强子，它概括了该基因在 T2 和 T3 腿中的时间和区域活性。然后，我们在 T2 腿细胞的可及染色质区域中使用转录因子 (TF) 结合基序分析来预测和功能测试可能调节 Ubx 腿增强子的 TF。我们还测试了 Ubx 辅助因子 Homothorax (Hth) 和 Extradenticle (Exd) 在 T2 和 T3 股骨中的作用。我们发现几个 TF 可能在上游或与 Ubx 协同作用，以调节发育中股骨近远端轴上的毛状体图案，并且毛状体的抑制也需要 Hth 和 Exd。总而言之，我们的结果提供了关于 Ubx 如何整合到胚胎后基因调控网络以确定精细腿部形态的见解。
Hox genes are expressed during embryogenesis and determine the regional identity of animal bodies along the antero-posterior axis. However, they also function post-embryonically to sculpt fine-scale morphology. To better understand how Hox genes are integrated into post-embryonic gene regulatory networks, we further analysed the role and regulation of Ultrabithorax (Ubx) during leg development in Drosophila melanogaster. Ubx regulates several aspects of bristle and trichome patterning on the femurs of the second (T2) and third (T3) leg pairs. We found that repression of trichomes in the proximal posterior region of the T2 femur by Ubx is likely mediated by activation of the expression of microRNA-92a and microRNA-92b by this Hox protein. Furthermore, we identified a novel enhancer of Ubx that recapitulates the temporal and regional activity of this gene in T2 and T3 legs. We then used transcription factor (TF) binding motif analysis in regions of accessible chromatin in T2 leg cells to predict and functionally test TFs that may regulate the Ubx leg enhancer. We also tested the role of the Ubx co-factors Homothorax (Hth) and Extradenticle (Exd) in T2 and T3 femurs. We found several TFs that may act upstream or in concert with Ubx to modulate trichome patterning along the proximo-distal axis of developing femurs and that the repression of trichomes also requires Hth and Exd. Taken together our results provide insights into how Ubx is integrated into a post-embryonic gene regulatory network to determine fine-scale leg morphology.
发育鲁棒性：果蝇中的吊带案。
影响因子：
5.5
作者：
Giraud G;Paul R;Duffraisse M;Khan S;Shashidhara LS;Merabet S
通讯作者：
Merabet S