Eugenol attenuates concanavalin A-induced hepatitis through modulation of cytokine levels and inhibition of mitochondrial oxidative stress
Eugenol attenuates concanavalin A-induced hepatitis through modulation of cytokine levels and inhibition of mitochondrial oxidative stress
复制标题
丁子香酚通过调节细胞因子水平和抑制线粒体氧化应激来减轻刀豆球蛋白 A 诱导的肝炎
DOI：
10.2298/abs190121016l
复制
发表时间：
2019-01-01
影响因子：
0.8
通讯作者：
Mao, Yidong
中科院分区：
文献类型：
--
作者：
Liu, Jing;Mao, Yidong
由于持续的氧化应激，单用常规药物治疗肝炎会长期损害肝脏功能。已经研究了来自几种植物来源的活性化合物来对抗这种情况。丁香酚是一种在各种植物中含量丰富的植物化学物质，以其广泛的药理作用而闻名。在分子水平上对其肝保护活性的深入理解存在空白。我们目前的研究旨在确定丁香酚对免疫介导的肝炎中抗氧化成分、炎性细胞因子和线粒体氧化应激调节的影响。我们采用了一种模型，模拟病毒性肝炎，使用刀豆球蛋白A（ConA）诱导T细胞介导的急性肝炎。丁香酚增加（P<0.01）抗氧化酶活性，包括还原型谷胱甘肽（GSH）再生酶，谷胱甘肽还原酶，葡萄糖-6-磷酸脱氢酶。白细胞介素和肿瘤坏死因子水平明显降低（P<0.01）。丁香酚被发现可以降低线粒体氧化应激，这在肝炎中升高。丁香酚的肝保护作用由组织学结果证实。目前的研究表明，丁香酚通过调节不同的途径发挥保肝作用，包括恢复线粒体氧化应激。丁香酚可能是一个有前途的候选人，人类肝炎管理，临床前研究。
Therapeutic management of hepatitis with conventional drugs alone worsens hepatic functioning in the long term because of sustained oxidative stress. Active compounds from several plant sources have been investigated to counteract this. Eugenol, a phytochemical abundant in various plants, is known for its wide range of pharmacological effects. There is a lacuna in the deeper understanding of its hepatoprotective activity at the molecular level. Our present study aimed to determine the effects of eugenol on the changes in antioxidant components, inflammatory cytokines and modulation of mitochondrial oxidative stress in immune-mediated hepatitis. We employed a model that mimics viral hepatitis using concanavalin A (ConA) to induce T-cell-mediated acute hepatitis. Eugenol increased (P<0.01) antioxidant enzyme activities, including reduced glutathione (GSH)-regenerating enzyme, glutathione reductase, and glucose-6-phosphate dehydrogenase. Its antiinflammatory and antifibrogenic effects were evident from the reduction (P<0.01) in interleukin and tumor necrosis factor levels. Eugenol was found to decrease mitochondrial oxidative stress, which was elevated in hepatitis. The hepatoprotective effects of eugenol were confirmed by histological findings. The current investigation shows that eugenol exerts a hepatoprotective effect through the modulation of different pathways which include restoration of mitochondrial oxidative stress. Eugenol could be a promising candidate for human hepatitis management, warranting preclinical studies.