An immunodominant domain in adenovirus type 2 early region 1A proteins.
An immunodominant domain in adenovirus type 2 early region 1A proteins.
复制标题
2 型腺病毒早期 1A 区蛋白中的免疫显性结构域。
DOI：
10.1128/jvi.60.1.312-316.1986
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发表时间：
1986
影响因子：
5.4
通讯作者：
Berk,AJ
中科院分区：
文献类型：
--
作者：
Tsukamoto,AS;Ferguson,B;Rosenberg,M;Weissman,IL;Berk,AJ
分离出六种独立的大鼠杂交瘤细胞系，它们产生针对人 C 亚型腺病毒早期区域 1A (E1A) 蛋白的单克隆抗体。竞争结合实验表明，每种单克隆抗体都针对 E1A 蛋白上的相同表位或重叠表位簇。使用病毒 E1A 缺失突变体和大肠杆菌中表达的 E1A 蛋白缺失形式将抗体识别位点定位到氨基酸 23 和 120 之间的序列，该序列编码在 E1A 基因的第一个外显子内。类似地，针对 trpE-E1A 融合蛋白以及针对天然生物活性 E1A 蛋白产生的多克隆抗血清也主要针对该免疫优势区域。
Six independent rat hybridoma cell lines producing monoclonal antibodies to human subgroup C adenovirus early region 1A (E1A) proteins were isolated. Competition binding experiments revealed that each of the monoclonal antibodies was directed against the same epitope or overlapping cluster of epitopes on the E1A proteins. Viral E1A deletion mutants and deleted forms of E1A proteins expressed in Escherichia coli were used to localize the antibody recognition sites to sequences between amino acids 23 and 120, encoded within the first exon of the E1A gene. Similarly, polyclonal antisera raised against the trpE-E1A fusion protein, as well as against the native, biologically active E1A protein, were also directed primarily against this immunodominant region.