Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles.
Measurements of the proton motive force generated by cytochrome c oxidase from Bacillus subtilis in proteoliposomes and membrane vesicles.
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测量蛋白脂质体和膜囊泡中枯草芽孢杆菌细胞色素 C 氧化酶产生的质子动力。
DOI：
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
W. Konings
中科院分区：
文献类型：
--
作者：
W. Vrij;A. Driessen;K. J. Hellingwerf;W. Konings
将枯草芽孢杆菌细胞色素C氧化酶重组到脂质体中，并对其能量传递特性进行了研究。所使用的重建程序包括钙离子诱导预成膜的融合。酶在脂质体中的定位受膜的磷脂组成的影响。带负电荷的磷脂对于高的氧化酶活性和呼吸控制是必不可少的。凝胶过滤、密度梯度离心法和电子显微镜分析表明，蛋白质脂质体在大小和呼吸控制方面具有异质性。蛋白脂质体中的细胞色素c氧化酶活性导致质子动力的产生，内部为负的和碱性的。在电子供体抗坏血酸/N，N，N‘，N’-四甲基对苯二胺/细胞色素c或抗坏血酸/吩嗪甲硫酸盐存在下，重组酶产生的电势为84 mV，加入黑素到95 mV时，产生的pH梯度为32 mV，加入瓦林霉素到39 mV时，pH梯度增加。牛心细胞色素C氧化酶在脂质体中的重组也得到了类似的结果。在没有内源离子泄漏的情况下，可产生的最大质子动力在110-140 mV范围内变化。由此计算出细胞色素C氧化酶的能量转导效率为18-23%，表明该酶是一种有效的质子动力产生系统。
Cytochrome c oxidase from Bacillus subtilis was reconstituted in liposomes and its energy-transducing properties were studied. The reconstitution procedure used included Ca2+-induced fusion of pre-formed membranes. The orientation of the enzyme in liposomes is influenced by the phospholipid composition of the membrane. Negatively charged phospholipids are essential for high oxidase activity and respiratory control. Analyses of the proteoliposomes by gel filtration, density gradient centrifugation and electron microscopy indicated a heterogeneity of the proteoliposomes with respect to size and respiratory control. Cytochrome c oxidase activity in the proteoliposomes resulted in the generation of a proton motive force, internally negative and alkaline. In the presence of the electron donor, ascorbate/N,N,N',N'-tetramethyl-p-phenylenediamine/cytochrome c or ascorbate/phenazine methosulphate, the reconstituted enzyme generated an electrical potential of 84 mV which was increased by the addition of nigericin to 95 mV and a pH gradient of 32 mV which was increased by the addition of valinomycin to 39 mV. Similar results were obtained with beef-heart cytochrome c oxidase reconstituted in liposomes. The maximal proton motive force which could be generated, assuming no endogenous ion leakage, varied over 110-140 mV. From this the efficiency of energy transduction by cytochrome c oxidase was calculated to be 18-23%, indicating that the oxidase is an efficient proton-motive-force-generating system.
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影响因子：
2.9
作者：
STRUCK, DK;HOEKSTRA, D;PAGANO, RE
通讯作者：
PAGANO, RE
细胞色素氧化酶的质子易位化学计量：使用快速响应的氧电极。
DOI：
10.1073/pnas.79.23.7218
发表时间：
1982
影响因子：
11.1
作者：
Reynafarje,B;Alexandre,A;Davies,P;Lehninger,AL
通讯作者：
Lehninger,AL
球形红假单胞菌细胞色素 c 氧化酶的纯化和表征。
DOI：
10.1111/j.1432-1033.1982.tb06667.x
发表时间：
1982
期刊：
European journal of biochemistry
影响因子：
--
作者：
Gennis,RB;Casey,RP;Azzi,A;Ludwig,B
通讯作者：
Ludwig,B
嗜热栖热菌细胞色素 c1aa3 复合物的细胞色素 c 氧化酶活性研究。
DOI：
--
发表时间：
1984
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Yoshida,T;Fee,JA
通讯作者：
Fee,JA