PBCAP380, a novel oocyst capsule protein, is essential for malaria parasite survival in the mosquito
PBCAP380, a novel oocyst capsule protein, is essential for malaria parasite survival in the mosquito
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DOI：
10.1111/j.1462-5822.2008.01127.x
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发表时间：
2008-06-01
影响因子：
3.4
通讯作者：
Jacobs-Lorena, Marcelo
中科院分区：
文献类型：
--
作者：
Srinivasan, Prakash;Fujioka, Hisashi;Jacobs-Lorena, Marcelo
传播疟疾病原体疟原虫的一个基本条件是蚊子媒介成功完成一个复杂的发育周期。在蚊子中肠中形成的数百个动合子中，只有少数转化为卵囊，这是蚊子免疫系统作用的损失。然而，一旦卵囊形成，它们似乎对蚊子的防御有抵抗力。在卵囊发育过程中，寄生虫周围形成一层厚厚的胶囊，似乎起着保护罩的作用。关于这种胶囊的组成信息很少。在这里，我们报告的第一个疟原虫卵囊蛋白（PbCap 380）的鉴定和部分表征。遗传分析表明，该基因是必不可少的，PbCap 380（-）突变体寄生虫形成正常数量的卵囊，但逐渐消除。因此，感染PbCap 380（-）寄生虫的蚊子不会传播疟疾。以卵囊为靶点可能为疟疾控制提供一种新的策略。
An essential requisite for transmission of Plasmodium, the causative agent of malaria, is the successful completion of a complex developmental cycle in its mosquito vector. Of hundreds of ookinetes that form in the mosquito midgut, only few transform into oocysts, a loss attributed to the action of the mosquito immune system. However, once oocysts form, they appear to be resistant to mosquito defences. During oocyst development, a thick capsule forms around the parasite and appears to function as a protective cover. Little information is available about the composition of this capsule. Here we report on the identification and partial characterization of the first Plasmodium oocyst capsule protein (PbCap380). Genetic analysis indicates that the gene is essential and that PbCap380(-) mutant parasites form oocysts in normal numbers but are gradually eliminated. As a result, mosquitoes infected with PbCap380(-) parasites do not transmit malaria. Targeting of the oocyst capsule may provide a new strategy for malaria control.