A bifunctional scaffold with CuFeSe2 nanocrystals for tumor therapy and bone reconstruction
A bifunctional scaffold with CuFeSe2 nanocrystals for tumor therapy and bone reconstruction
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用于肿瘤治疗和骨重建的具有 CuFeSe2 纳米晶体的双功能支架
DOI：
10.1016/j.biomaterials.2017.11.020
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发表时间：
2018-04-01
期刊：
影响因子：
14
通讯作者：
Wu, Chengtie
中科院分区：
文献类型：
--
作者：
Dang, Wentao;Li, Tao;Wu, Chengtie
骨肿瘤是临床上面临的重大挑战之一。手术干预后，一些骨肿瘤细胞仍然残留在骨缺损周围，然后在几天内增殖。制备具有骨肿瘤治疗和骨再生双重功能的特异性生物材料具有重要意义。为了实现这一目标，我们通过将3D打印技术与溶剂热方法相结合，成功制备了由CuFeSe2纳米晶功能化的生物活性玻璃（BG）支架（BG-CFS）。在溶剂热反应过程中，CuFeSe2纳米晶可以在BG支架的支柱表面原位生长，从而赋予BG支架优异的光热性能。通过改变 CuFeSe2 纳米晶体的含量和暴露于近红外激光（808 nm）时的激光功率密度，可以很好地调节 BG-CFS 支架的光热性能。 BG-CFS支架不仅能够在体外有效消融骨肿瘤细胞（Saos-2细胞），而且在体内也能显着抑制骨肿瘤生长。此外，BG-CFS支架可以刺激兔骨髓基质细胞（rBMSCs）的成骨基因表达，最终促进骨缺损处新骨的形成。我们的研究首次将半导体CuFeSe2纳米晶体的光热性能与生物活性玻璃支架的成骨活性结合起来，为开发具有骨肿瘤治疗和骨再生双重功能的新型生物材料提供了更广阔的视野。 (C) 2018 Elsevier Ltd. 保留所有权利。
Bone tumor is one of major challenging issues clinically. After surgical intervention, a few bone tumor cells still remain around bone defects and then proliferate over days. Fabrication of specific biomaterials with dual functions of bone tumor therapy and bone regeneration is of great significance. In order to achieve this aim, we managed to prepare bioactive glass (BG) scaffolds functionalized by the CuFeSe2 nanocrystals (BG-CFS) by combining 3D printing technique with solvothermal method. During the solvothermal reaction process, CuFeSe2 nanocrystals could in situ grow on the strut surface of BG scaffolds and thus endow BG scaffolds excellent photothermal performance. The photothermal performance of BG-CFS scaffolds could be well regulated through altering the content of CuFeSe2 nanocrystals and laser power density when exposed to the near infrared laser (808 nm). The BG-CFS scaffolds could not only effectively ablate the bone tumor cells (Saos-2 cells) in vitro, but also significantly inhibit bone tumor growth in vivo. Moreover, BG-CFS scaffolds could stimulate osteogenic gene expressions of rabbit bone marrow stromal cells (rBMSCs) and finally facilitate the formation of new bone in the bone defects. Our study, for the first time, combined the photothermal performance of semiconductor CuFeSe2 nano crystals with the bone-forming activity of bioactive glass scaffolds, which can offer a more extensive horizon for developing novel biomaterials with dual functions of bone tumor therapy and bone regeneration. (C) 2018 Elsevier Ltd. All rights reserved.