AN ANALYSIS OF COLLAGEN SECRETION BY ESTABLISHED MOUSE FIBROBLAST LINES.
AN ANALYSIS OF COLLAGEN SECRETION BY ESTABLISHED MOUSE FIBROBLAST LINES.
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DOI：
10.1083/jcb.22.1.227
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发表时间：
1964-07
期刊：
影响因子：
--
通讯作者：
GREEN H
中科院分区：
文献类型：
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作者：
GOLDBERG B;GREEN H
已建立的小鼠成纤维细胞系的胶原蛋白的体外合成已通过电子显微镜检查。在快速生长（对数期），当胶原蛋白不能检测到的文化，细胞缺乏一个发达的颗粒ergastoplasm和高尔基体系统。停止生长后（静止期），胶原蛋白在培养物中积累，细胞表现出高度发达的颗粒状和光滑的ergastoplasm。胶原蛋白似乎是在粗糙表面的内质网中合成的，并作为可溶性蛋白质通过无颗粒的ergastoplasm的囊泡元件运输到细胞表面。这些囊泡的界膜与细胞膜的融合允许可溶性胶原蛋白排放到细胞外空间，在那里形成两种直径分布的原纤维。胶原蛋白的分泌属于局部分泌型。胶原蛋白分泌的替代理论进行了讨论，并与其他在体外和体内研究的胶原纤维形成的结果相比，建立线的数据。
In vitro synthesis of collagen by established mouse fibroblast lines has been examined by electron microscopy. During rapid growth (log phase), when collagen could not be detected in the cultures, the cells lacked a well developed granular ergastoplasm and Golgi system. Upon cessation of growth (stationary phase), collagen accumulated in the cultures and the cells demonstrated highly developed granular and smooth ergastoplasm. Collagen appeared to be synthesized in the rough-surfaced endoplasmic reticulum and to be transported as a soluble protein to the cell surface by vesicular elements of the agranular ergastoplasm. Fusion of the limiting membranes of these vesicles with the cell membrane permitted the discharge of the soluble collagen into the extracellular space, where fibrils of two diameter distributions formed. The secretion of collagen is concluded to be of the merocrine type. Alternative theories of collagen secretion are discussed and the data for established lines compared with the results of other in vitro and in vivo studies of collagen fibrillogenesis.