Antioxidant cellulose nanofibers/lignin-based aerogels: a potential material for biomedical applications
Antioxidant cellulose nanofibers/lignin-based aerogels: a potential material for biomedical applications
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DOI：
10.1186/s40538-023-00438-z
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发表时间：
2023-08-07
影响因子：
6.6
通讯作者：
Rodriguez, Alejandro
中科院分区：
文献类型：
--
作者：
Sanchez, Laura M.;Hopkins, Abigail K.;Rodriguez, Alejandro
木质素是一种天然存在的芳香生物聚合物，具有众所周知的抗菌和抗氧化特性。因此，在这项工作中，研究了利用纤维素纳米纤维（CNF）和木质素生产用于生物医学应用的超轻气凝胶。气凝胶含有不同数量的木质素（0-30 wt%）和不同浓度的交联剂Fe3+ (25-100 mM)。结果对不同生物气凝胶进行了全面表征，并对其物理、力学和生物活性进行了分析。结果表明，在不同Fe3+浓度下，随着木质素含量的增加，生物气凝胶的可溶组分呈下降趋势，这是由于木质素与cnf通过氢键相互作用所致。随着DPPH浓度的降低，木质素生物气凝胶具有明显的自由基清除活性。这证实了在生物气凝胶中加入木质素以赋予抗氧化性能的好处。为了研究制备的生物气凝胶对药物控释的适宜性，对四环素（TC）的释放进行了研究。所有生物凝胶均能持续释放TC 6 h，且表现出相似的特征。而交联剂浓度越高的生物气凝胶，TC的释放量越大。与不含TC的生物气凝胶相比，负载TC的生物气凝胶对金黄色葡萄球菌具有明显的抗菌活性。通过多次比较的Kruskal-Wallis测试证明了样品的生物相容性，适用于所有生产的材料（有和没有TC加载）。在观察细胞形态后，没有明显的差异，这表明cnf -木质素生物气凝胶具有最佳的生物相容性，可用于生物医学和制药行业。结论cnf -木质素生物气凝胶由于其生物相容性、抗菌性、抗氧化性以及溶胀性等特点，在伤口敷料等生物医学领域具有广阔的应用前景。
Background Lignin is a naturally occurring and aromatic biopolymer with well-known antimicrobial and antioxidant properties. Thus, in this work, the use of cellulose nanofibers (CNF) and lignin to produce ultra-light aerogels for biomedical applications was studied. Aerogels containing varying amounts of lignin (0-30 wt%) and different concentrations of the crosslinking agent Fe3+ (25-100 mM) were developed.Results The different bioaerogels were fully characterized and their physical, mechanical and bioactive properties analyzed. It was observed that the bioaerogels soluble fraction tends to decrease as the lignin content increases for the different Fe3+ concentrations, due to lignin-CNF interactions through hydrogen bonds. The bioaerogels containing lignin showed remarkable radical scavenging activity as the DPPH concentration decreased with time. This confirms the benefits of including lignin in bioaerogels to impart antioxidant properties. To study the suitability of the produced bioaerogels for controlled drug release, the release of tetracycline (TC) was studied. All of the bioaerogels released TC in a sustained manner for 6 h and presented similar profiles. However, the bioaerogels containing higher concentrations of crosslinker showed a higher release of TC. The TC loading conferred clear antimicrobial activity against S. aureus as expected, unlike the insignificant antimicrobial activity of the bioaerogels without TC. The biocompatibility of the samples was demonstrated for all materials produced (with and without TC loading) by the Kruskal-Wallis test with multiple comparisons. After observation of cell morphology, no significant differences were evident suggesting that the CNF-lignin bioaerogels present optimal biocompatibility for use in the biomedical and pharmaceutical industry.Conclusions The CNF-lignin bioaerogels presented in this work highlights their promising application as biomedical applications, such as wound dressings due to their biocompatibility, antimicrobial and antioxidant properties, as well as their swelling and solubility properties.