Possible functions of Dpp in gastropod shell formation and shell coiling
Possible functions of Dpp in gastropod shell formation and shell coiling
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DOI：
10.1007/s00427-011-0358-4
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发表时间：
2011-06-01
影响因子：
2.4
通讯作者：
Endo, Kazuyoshi
中科院分区：
文献类型：
--
作者：
Shimizu, Keisuke;Sarashina, Isao;Endo, Kazuyoshi
为了了解腹足类动物的发育机制和壳形成的进化过程，我们检测了dpp在肺蜗牛lynaea滞螺中的表达模式，并利用dpp信号抑制剂dorsomorphin分析了dpp的功能。dpp基因在右旋螺的齿状体期表达于壳腺周围圆形区域的右半部分，在螺壳期表达于地幔的右侧。当Dpp信号被dorsomorphin抑制时，观察到两种类型的外壳畸形。在壳腺形成前的2细胞期和囊胚期用dorsomorphin处理，幼胚壳发育不完全，未矿化。另一方面，当在壳腺形成后的齿状体和棘状体阶段处理时，幼螺的壳发育成圆锥形，而不是正常的卷曲状。这些结果表明，dpp在这种腹足动物的壳的形成和盘绕中起重要作用。
We examined dpp expression patterns in the pulmonate snail Lymnaea stagnalis and analyzed the functions of dpp using the Dpp signal inhibitor dorsomorphin in order to understand developmental mechanisms and evolution of shell formation in gastropods. The dpp gene is expressed in the right half of the circular area around the shell gland at the trochophore stage and at the right-hand side of the mantle at the veliger stage in the dextral snails. Two types of shell malformations were observed when the Dpp signals were inhibited by dorsomorphin. When the embryos were treated with dorsomorphin at the 2-cell and blastula stages before the shell gland is formed, the juvenile shells grew imperfectly and were not mineralized. On the other hand, when treated at the trochophore and veliger stage after the shell gland formation, juvenile shells grew to show a cone-like form rather than a normal coiled form. These results indicated that dpp plays important roles in the formation and coiling of the shell in this gastropod species.