Hemoglobin adducts of benzo[a]pyrene diolepoxide in newspaper vendors: Association with traffic exhaust
Hemoglobin adducts of benzo[a]pyrene diolepoxide in newspaper vendors: Association with traffic exhaust
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DOI：
10.1093/carcin/17.11.2389
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发表时间：
1996-11-01
期刊：
影响因子：
4.7
通讯作者：
Airoldi, L
中科院分区：
文献类型：
--
作者：
Pastorelli, R;Restano, J;Airoldi, L
用气相色谱-质谱法测定汽车尾气中苯并[a]芘二醇环氧化物与血红蛋白(Hb)的加合物，用气相色谱-质谱法测定汽车尾气酸解后释放的苯并[a]芘四氢四氢吡喃(BPTs)，经免疫亲和层析后，对53名报贩进行检测，高密度交通接触者BPTs/mg Hb中位浓度为0.3fmolBPTs/mg Hb，低密度交通接触者小于或等于0.1fmolBPTs/mg Hb，差异无统计学意义(P=0.09)。在非吸烟者中，60%的高暴露受试者(中位数0.3fmolBPTs/mg Hb)和28%的低暴露受试者(中位数小于或等于0.1fmolBPTs/mg Hb)可检测到加合物。差异有统计学意义(P=0.02)。低暴露吸烟者加合物的中位数为0.26fmolBPTs/mg Hb，而低暴露非吸烟者的加合物中位数小于或等于0.1fmolBPTs/mg Hb(P=0.08，P<0.05)。低密度和高密度交通暴露吸烟者的加合物浓度无差异(P=0.82)。数据表明，非吸烟者在与交通废气暴露相关的加合物浓度方面存在显著差异。
Benzo[a]pyrene diol epoxide adducts with hemoglobin (Hb) were measured to detect human exposure to environmental benzo[a]pyrene from traffic exhaust, Benzo[a]pyrene tetrahydrotetrols (BPTs) released from uh after acid hydrolysis were quantitated by gas chromatography-mass spectrometry after immunoaffinity chromatography, Fifty three newspaper vendors were enrolled, The median adduct concentration was 0.3 fmol BPTs/mg Hb in high density traffic-exposed vendors and less than or equal to 0.1 fmol BPTs/mg Hb in those exposed to low density traffic; the difference was not significant (P=0.09). Among non-smokers, adducts were detectable in 60% of high exposure subjects (median 0.3 fmol BPTs/mg Hb) and in 28% of those with low exposure (median less than or equal to 0.1 fmol/mg Hb). This difference was significant (P=0.02). In low exposure smokers the median of adducts was 0.26 fmol BPTs/mg Hb, while in low exposure non-smokers it was less than or equal to 0.1 fmol BPTs/mg Hb (P=0.08, not significant). Adduct concentration was no different for low and high density traffic-exposed smokers (P=0.82). The data indicate a significant difference in adduct concentration related to traffic exhaust exposure among non-smokers.