Sulfur-Doped Graphene Quantum Dots as a Novel Fluorescent Probe for Highly Selective and Sensitive Detection of Fe3+
Sulfur-Doped Graphene Quantum Dots as a Novel Fluorescent Probe for Highly Selective and Sensitive Detection of Fe3+
复制标题
掺硫石墨烯量子点作为新型荧光探针，用于高选择性和高灵敏度检测 Fe3
DOI：
10.1021/ac503183y
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发表时间：
2014-10-21
影响因子：
7.4
通讯作者：
Li, Xiaohong
中科院分区：
文献类型：
--
作者：
Li, Shuhua;Li, Yunchao;Li, Xiaohong
通过石墨在对甲苯磺酸钠水溶液中的一步电解合成了具有稳定蓝绿色荧光的硫掺杂石墨烯量子点（S-GQD）。与GQDs相比，S-GQDs显着提高了电子性能和表面化学反应活性，对Fe3+表现出敏感的响应。因此，S-GQDs 被用作高效荧光探针，用于高选择性检测 Fe3+。随着Fe3+浓度在0.01~0.70 μM范围内增加，S-GQDs的荧光强度逐渐降低，并在0.90 μM处达到稳定水平。添加Fe3+前后S-GQDs的荧光强度差异与Fe3+浓度成正比，校准曲线在0-0.70 μM范围内呈线性区域。检测限为4.2 nM。最后，这种新型荧光探针成功应用于人血清中Fe3+的直接分析，在临床诊断中具有潜在的应用前景，并可能为其他生物学相关靶点的有效荧光探针的设计开辟新的途径。
Sulfur-doped graphene quantum dots (S-GQDs) with stable blue-green fluorescence were synthesized by one-step electrolysis of graphite in sodium p-toluenesulfonate aqueous solution. Compared with GQDs, the S-GQDs drastically improved the electronic properties and surface chemical reactivities, which exhibited a sensitive response to Fe3+. Therefore, the S-GQDs were used as an efficient fluorescent probe for highly selective detection of Fe3+. Upon increasing of Fe3+ concentration ranging from 0.01 to 0.70 mu M, the fluorescence intensity of S-GQDs gradually decreased and reached a plateau at 0.90 mu M. The difference in the fluorescence intensity of S-GQDs before and after adding Fe3+ was proportional to the concentration of Fe3+, and the calibration curve displayed linear regions over the range of 0-0.70 mu M. The detection limit was 4.2 nM. Finally, this novel fluorescent probe was successfully applied to the direct analysis of Fe3+ in human serum, which presents potential applications in clinical diagnosis and may open a new way to the design of effective fluorescence probes for other biologically related targets.